MABS1304
Anti-ATP Synthase subunit β Antibody, clone 11/21-7-A8
clone 11/21-7-A8, from mouse
Synonym(s):
ATP synthase subunit beta, mitochondrial, ATP Synthase subunit β, beta-F1-ATPase
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All Photos(2)
About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
mouse
Quality Level
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
11/21-7-A8, monoclonal
species reactivity
human, mouse, rat
technique(s)
ELISA: suitable
dot blot: suitable
immunocytochemistry: suitable
western blot: suitable
isotype
IgG1κ
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... ATP5B(506)
General description
ATP synthase subunit beta, mitochondrial (EC 3.6.3.14; UniProt P06576; also known as ATP synthase H+ transporting mitochondrial F1 complex beta polypeptide, beta-F1-ATPase, Epididymis secretory protein Li 271, Mitochondrial ATP synthase beta subunit, Mitochondrial ATP synthetase beta subunit) is encoded by the ATP5B (also known as ATPMB, ATPSB, HEL-S-271) gene (Gene ID 506) in human. Mitochondrial ATP synthase produces ATP from ADP in the presence of a proton gradient generated by electron transport complexes. This ATPase contains two structural domains, F1-containing extramembrane catalytic core, and F0-containing the membrane proton channel that are linked via a central stalk and a peripheral stalk. Subunits alpha and beta form the catalytic code in F1. Tumor development requires the selection of cancer cells with a repressed biogenesis and functional activity of mitochondria. Both beta-F1-ATPase/GAPDH and beta-F1-ATPase/Hsp60 ratios are found to be significantly lower in tumors than the corresponding normal tissues. Studies conducted in human colon cancer cell line HCT116 show the involvement of AMPK (AMP-activated protein kinase) and GCN2 (general control non-derepressible 2; eIF2α kinase) in the onset of colon cancer progression by repressing of beta-F1-ATPase synthesis and promoting the abnormal bioenergetics of mitochondria.
Immunogen
His-tagged recombinant protein corresponding to human ATP Synthase subunit β.
Application
Immunocytochemistry Analysis: A representative lot immunostained mitochondrial tubular network in human breast cancer Hs578T cells (Acebo, P., et al (2009). Transl Oncol. 2(3):138-145).
ELISA Analysis: A representative lot detected His-tagged full-length human ATP Synthase subunit β (beta-F1-ATPase) recombinant protein by direct ELISA (Acebo, P., et al (2009). Transl Oncol. 2(3):138-145).
Dot Blot Analysis: A representative lot detected ATP Synthase subunit β (beta-F1-ATPase) by Dot blot using His-tagged full-length human beta-F1-ATPase recombinant protein or HepG2 lysate (Acebo, P., et al (2009). Transl Oncol. 2(3):138-145).
Western Blotting Analysis: A representative lot detected ATP Synthase subunit β (beta-F1-ATPase) in human hepatoma HepG2, murine hepatoma Hepa 1-6, and normal rat liver epithelial C9 (Clone 9) cells.
Western Blotting Analysis: A representative lot detected ATP Synthase subunit β (beta-F1-ATPase) expression in various cancer patients tissues (Acebo, P., et al (2009). Transl Oncol. 2(3):138-145).
Western Blotting Analysis: A representative lot detected ATP Synthase subunit β (beta-F1-ATPase) downregulation in HCT116 human colon cancer cells in response to AMPK pathway activation upon oligomycin or AICAR treatment (Martinez-Reyes, J., et al. (2012). Biochem J. 444(2):249-259).
ELISA Analysis: A representative lot detected His-tagged full-length human ATP Synthase subunit β (beta-F1-ATPase) recombinant protein by direct ELISA (Acebo, P., et al (2009). Transl Oncol. 2(3):138-145).
Dot Blot Analysis: A representative lot detected ATP Synthase subunit β (beta-F1-ATPase) by Dot blot using His-tagged full-length human beta-F1-ATPase recombinant protein or HepG2 lysate (Acebo, P., et al (2009). Transl Oncol. 2(3):138-145).
Western Blotting Analysis: A representative lot detected ATP Synthase subunit β (beta-F1-ATPase) in human hepatoma HepG2, murine hepatoma Hepa 1-6, and normal rat liver epithelial C9 (Clone 9) cells.
Western Blotting Analysis: A representative lot detected ATP Synthase subunit β (beta-F1-ATPase) expression in various cancer patients tissues (Acebo, P., et al (2009). Transl Oncol. 2(3):138-145).
Western Blotting Analysis: A representative lot detected ATP Synthase subunit β (beta-F1-ATPase) downregulation in HCT116 human colon cancer cells in response to AMPK pathway activation upon oligomycin or AICAR treatment (Martinez-Reyes, J., et al. (2012). Biochem J. 444(2):249-259).
Research Category
Signaling
Signaling
Research Sub Category
Developmental Signaling
Developmental Signaling
This Anti-ATP Synthase subunit β Antibody, clone 11/21-7-A8 is validated for use in Western Blotting, Immunocytochemistry, ELISA and Dot Blot for the detection of ATP Synthase subunit β.
Quality
Evaluated by Western Blotting in HepG2 cell lysate.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected ATP Synthase subunit β in 10 µg of HepG2 cell lysate.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected ATP Synthase subunit β in 10 µg of HepG2 cell lysate.
Target description
~56 kDa observed
Physical form
Format: Purified
Protein G Purified
Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Other Notes
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Regulatory Listings
Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.
JAN Code
MABS1304:
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Find documentation for the products that you have recently purchased in the Document Library.
Cancer abolishes the tissue type-specific differences in the phenotype of energetic metabolism.
Acebo, P; Giner, D; Calvo, P; Blanco-Rivero, A; Ortega, AD; Fernandez, PL; Roncador et al.
Translational Oncology null
AMPK and GCN2-ATF4 signal the repression of mitochondria in colon cancer cells.
Martinez-Reyes, I; Sanchez-Arago, M; Cuezva, JM
The Biochemical Journal null
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