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Safety Information

06-852

Sigma-Aldrich

Anti-Spinophilin/Neurabin-II Antibody

Upstate®, from rabbit

Synonym(s):

Protein phosphatase 1 regulatory subunit 9B 3 neurabin II, protein phosphatase 1, regulatory (inhibitor) subunit 9B, protein phosphatase 1, regulatory subunit 9B, protein phosphatase 1, regulatory subunit 9B, spinophilin

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

100

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

polyclonal

species reactivity

rat

manufacturer/tradename

Upstate®

technique(s)

immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

isotype

IgG

NCBI accession no.

NM_032595

UniProt accession no.

Q96SB3

shipped in

dry ice

target post-translational modification

unmodified

Gene Information

human ... PPP1R9B(84687)

General description

Spinophilin/neurabin II is an actin-associated scaffolding protein enriched in the dendritic spines of neurons.

Specificity

Other species not known.
Specific for spinophilin, Mr 120 kDa. Also recognizes a band which is suspected to be a cleavage product of spinophilin, Mr 95 kDa.

Immunogen

GST fusion protein corresponding to residues 286-390 of rat brain spinophilin/neurabin-II.

Application

Anti-Spinophilin/Neurabin-II Antibody is an antibody against Spinophilin/Neurabin-II for use in IC, IP & WB.
Immunoprecipitation:
4 μg of a previous lot immunoprecipitated Spinophilin/Neurabin-II from 500 μg of rat brain microsomal preparation (Catalog # 12-144).
Research Category
Neuroscience
Research Sub Category
Synapse & Synaptic Biology

Neuronal & Glial Markers

Quality

Routinely evaluated by Western Blot on a rat brain microsomal preparation.

Western Blot Analysis:
0.25-1 μg/mL of this lot detected Spinophilin/Neurabin-II in 20 μg of a rat brain microsomal preparation (Catalog # 12-144).

Target description

120 kDa

Physical form

Format: Purified
Protein G Purified
Purified rabbit polyclonal IgG in buffer containing 70% storage buffer (0.014 M phosphate buffer, pH 7.6, 0.175 M NaCl, 0.07% sodium azide) and 30% glycerol. Store at -20°C.

Storage and Stability

Stable for 1 year at -20ºC from date of receipt.

Handling Recommendations:
Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerolcontaining solutions to become frozen during storage.

Analysis Note

Control
Brain tissue

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

06-852:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Interaction of rat lin-10 with brain-enriched F-actin-binding protein, neurabin-II/spinophilin.
Ide, N, et al.
Biochemical and biophysical research communications, 244, 258-262 (1998)
Bridget M Nugent et al.
Nature neuroscience, 18(5), 690-697 (2015-03-31)
The developing mammalian brain is destined for a female phenotype unless exposed to gonadal hormones during a perinatal sensitive period. It has been assumed that the undifferentiated brain is masculinized by direct induction of transcription by ligand-activated nuclear steroid receptors.
Ryan T Terry-Lorenzo et al.
The Journal of biological chemistry, 277(48), 46535-46543 (2002-09-25)
Inhibitor-2 (I-2) bound protein phosphatase-1 (PP1) and several PP1-binding proteins from rat brain extracts, including the actin-binding proteins, neurabin I and neurabin II. Neurabins from rat brain lysates were sedimented by I-2 and its structural homologue, I-4. The central domain
A Satoh et al.
The Journal of biological chemistry, 273(6), 3470-3475 (1998-03-07)
In a preceding paper, we reported a novel actin filament (F-actin)-binding protein, named neurabin, which was specifically expressed in neural tissue and implicated in neurite formation. We purified from rat brain another F-actin-binding protein, which had a domain organization similar
Age-related changes in Usp9x protein expression and DNA methylation in mouse brain.
Jun Xu
Brain Research Molecular Brain Research null
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