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06-702

Sigma-Aldrich

Anti-phospho-Smad1 (Ser463/465) Antibody

Upstate®, from rabbit

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

100

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

polyclonal

species reactivity

human, Xenopus, rat, mouse

manufacturer/tradename

Upstate®

technique(s)

western blot: suitable

isotype

IgG

NCBI accession no.

NM_005900

UniProt accession no.

Q15797

shipped in

dry ice

target post-translational modification

phosphorylation (pSer463/pSer465)

Gene Information

human ... SMAD1(4086)

General description

Smad proteins, the mammalian homologs of the Drosophila Mothers against dpp (Mad), function downstream of TGF-β receptor Serine/Threonine kinases and undergo serine phosphorylation in response to receptor activation. Following BMP (bone morphogenic protein) or TGF-β binding to the targeted surface receptors, Smad1 (also designated Madr1 or JV4-1) becomes phosphorylated at Ser 463 and Ser 465. Activated Smad1 associates with Smad4 and translocates to the nucleus, where the Smad1/Smad4 complex recruits DNA-binding proteins to activate specific gene transcription. BMP binding induces phosphorylation of Smad1, which enhances the binding of Smad1 to CBP to stimulate Smad1-dependent transcription. In addition, ALK1 and ALK2 specifically phosphorylate Smad1 on residues located in the MH2 domain and induces Smad1-dependent pathways.

Specificity

Recognizes the dual serine phosphorylated Smad1 (Ser463/465).

Immunogen

peptide corresponding to (CGGSPHNPISpSVpSGG) corresponding to amino acids 455-465 of human Smad1 protein

Application

Detect phospho-Smad1 (Ser463/465) with Anti-phospho-Smad1 (Ser463/465) Antibody (Rabbit Polyclonal Antibody), that has been shown to work in WB.

Quality

routinely evaluated by immunoblot on RIPA lysates from P19 (mouse embryonal carcinoma) cells treated with BMP4

Target description

65 kDa

Physical form

Format: Purified

Analysis Note

Control
BMP-4-treated HeLa or NIH/3T3 cells

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

06-702:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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BMP signaling dynamics in embryonic orofacial tissue.
Partha Mukhopadhyay,Cynthia L Webb,Dennis R Warner,Robert M Greene,M Michele Pisano
Journal of Cellular Physiology null
Signal transduction. Mad about SMADs.
J Wrana et al.
Nature, 388(6637), 28-29 (1997-07-03)
Interaction between Smad1 and p97/VCP in rat testis and epididymis during the postnatal development.
Sevil Cayli,Fikret Erdemir,Seda Ocakli,Bahadir Ungor,Hakan Kesici,Tamer Yener,Huseyin Aslan
Reproductive Sciences null
Reduced endoglin activity limits cardiac fibrosis and improves survival in heart failure.
Kapur, NK; Wilson, S; Yunis, AA; Qiao, X; Mackey, E; Paruchuri, V; Baker, C; Aronovitz et al.
Circulation null
Cross-talk between fibroblast growth factor and bone morphogenetic proteins regulates gap junction-mediated intercellular communication in lens cells.
Boswell, BA; Lein, PJ; Musil, LS
Molecular Biology of the Cell null
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