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ZRB1572

Sigma-Aldrich

Anti-pSmad1 Ser463/465 Antibody, clone 1N12 ZooMAb® Rabbit Monoclonal

enhanced validation
greener alternative

recombinant, expressed in HEK 293 cells

Synonym(s):

Mothers against decapentaplegic homolog 1

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.43

biological source

rabbit

Quality Level

recombinant

expressed in HEK 293 cells

conjugate

unconjugated

antibody form

purified antibody

antibody product type

primary antibodies

clone

1N12, recombinant monoclonal

description

recombinant, expressed in HEK 293 cells

product line

ZooMAb® learn more

form

lyophilized

mol wt

calculated mol wt 52.26 kDa
observed mol wt ~55 kDa

purified by

using Protein A

species reactivity

human

species reactivity (predicted by homology)

monkey, horse, canine, mouse, feline, chicken, rat, bovine

packaging

antibody small pack of 25 μL

greener alternative product characteristics

Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.

enhanced validation

recombinant expression
Learn more about Antibody Enhanced Validation

sustainability

Greener Alternative Product

technique(s)

affinity binding assay: suitable
peptide synthesis: suitable
western blot: suitable

isotype

IgG

epitope sequence

C-terminal

Protein ID accession no.

UniProt accession no.

greener alternative category

shipped in

ambient

storage temp.

2-8°C

target post-translational modification

phosphorylation (pSer463/pSer465)

Gene Information

human ... SMAD1(4086)

General description

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies.

Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.

Specificity

Clone 1N12 is a ZooMAb® Rabbit recombinant monoclonal antibody that specifically detects Smad1 phosphorylated on serine 463/465.

Immunogen

KLH-conjugated linear peptide corresponding to 11 amino acids surrounding phosphoserine 463/465 from the C-terminal region of human Smad1.

Application

Quality Control Testing

Evaluated by Western Blotting in Jurkat cells treated with BMP2.

Western Blotting Analysis (WB): A 1:1,000 dilution of this antibody detected Smad1 phosphorylated on serine 463/465 in lysate from serum starved Jurkat cells treated with BMP2 (50 ng/mL; 30 min.).

Tested applications

Peptide Inhibition Assay: Target band detection in Lysate from HeLa cells treated with BMP2 was prevented by preblocking of a representative lot with the immunogen phosphopeptide, but not the corresponding non-phosphopeptide.

Affinity Binding Assay: A representative lot of this antibody bound phospho-Smad1-Ser463/465 with a KD of 2.4 x 10-7 in an affinity binding assay.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Target description

Mothers against decapentaplegic homolog 1 (UniProt: Q15797; also known as MAD homolog 1, Mothers against DPP homolog 1, JV4-1, Mad-related protein 1, SMAD family member 1, SMAD1, Smad1, hSMAD1, Transforming growth factor-beta-signaling protein 1, BSP-1) is encoded by the SMAD1 (also known as BSP1, MADH1, MADR1) gene (Gene ID: 4086) in human. Smad1 is a transcriptional modulator that is activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. Smad1 is a receptor-regulated Smad (R-Smad) that is found in a complex with Smad4 and YY1. Upon phosphorylation at the C-terminal SVS motif (Ser463/465) by BMP type 1 receptor kinase it forms a trimer with another Smad1 and Smd4. The C-terminal phosphoserines of Smad1 are recognized by the Mad Homology 2 (MH2) domain of Smad4. The Smad1/Smad4 complex then translocates to the nucleus where Smad proteins bind to their cognate DNA binding sites with low affinity. This binding is further enhanced in the presence of transcriptional co-activators. It is exported from the nucleus to the cytoplasm upon dephosphorylation. Smad1 is ubiquitinated by Smad-specific E3 ubiquitin ligase SMURF1, leading to its degradation via proteasome Smad1 variants are associated with susceptibility to pulmonary hypertension that is characterized by plexiform lesions of proliferating endothelial cells in pulmonary arterioles. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.

Physical form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

Reconstitution

300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

ZRB1572-25UL:
ZRB1572-4X25UL:


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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