Method optimization by solid-phase microextraction in combination with gas chromatography with mass spectrometry for analysis of beer volatile fraction.

Method optimization by solid-phase microextraction in combination with gas chromatography with mass spectrometry for analysis of beer volatile fraction.

Journal of chromatography. A (2006-05-12)

Olivia Pinho, Isabel M P L V O Ferreira, Lúcia H M L M Santos

ABSTRACT

A simple and sensitive method for the analysis of beer volatile compounds was optimised using headspace solid-phase microextraction (SPME) and gas chromatography with mass detection. Headspace SPME using a 75 microm Carboxen-polydimethylsiloxane (CAR-PDMS) fiber provided effective sample enrichment and enabled extraction of a wide variety of compounds. The reproducibility depended on the compounds, with a mean value of 1.4% for alcohols, 3.3% for ethers, 6.7% for aldehydes, 3.4% for acids, 1.7% for aromatic compounds, 2.4% for esters, 7.4% for hydrocarbons, 1.8% for alicyclic compounds, and 3.4% for heterocyclic compounds. The optimised methodology can be used to compare volatile profile from different types of beers and eventually to study the evolution of a particular beer during aging.

MATERIALI

N° Catalogo

Marchio

Descrizione del prodotto

57348-U

Supelco

Gruppo in fibra SPME divinilbenzene/carbossene/polidimetilsilossano (DVB/CAR/PDMS), needle size 24 ga, StableFlex

57328-U

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Gruppo in fibra SPME divinilbenzene/carbossene/polidimetilsilossano (DVB/CAR/PDMS), needle size 24 ga, for use with manual holder

57298-U

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Gruppo in fibra SPME divinilbenzene/carbossene/polidimetilsilossano (DVB/CAR/PDMS), needle size 23 ga, StableFlex, for use with autosampler

57299-U

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Gruppo in fibra SPME divinilbenzene/carbossene/polidimetilsilossano (DVB/CAR/PDMS), needle size 23 ga, StableFlex, for use with manual holder or autosampler, fiber L 2 cm

20358

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ORBO^™ 90 Carboxen^® 564 (20/45), 160/80 mg, W,F,F separators, O.D. × L 6 mm × 75 mm, pkg of 25 ea, for analyte group MEK (methylethyl ketone)

57329-U

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Gruppo in fibra SPME divinilbenzene/carbossene/polidimetilsilossano (DVB/CAR/PDMS), needle size 24 ga, StableFlex, for use with autosampler

20355

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ORBO^™ 78 HBr on Carboxen^® 564 specially cleaned (20/45), 400/200 mg, W,W,W separators, O.D. × L 6 mm × 110 mm, pkg of 25 ea

10478-U

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Adsorbente Carboxen^®, matrix Carboxen^® 1000, 60-80 mesh, bottle of 10 g

57318

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Gruppo in fibra SPME carbossene/polidimetilsilossano (CAR/PDMS), d_f 75 μm(CAR/PDMS, for use with manual holder, needle size 24 ga

25467

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Carboxen^®-1010 PLOT Capillary GC Column, L × I.D. 30 m × 0.53 mm, average thickness 30 μm

25461

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Colonna capillare per GC Carboxen^®-1006 PLOT, L × I.D. 30 m × 0.53 mm, average thickness 30 μm

57295-U

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Gruppo in fibra SPME carbossene/polidimetilsilossano (CAR/PDMS), d_f 85 μm(CAR/PDMS, needle size 23 ga, StableFlex, for use with autosampler

57335-U

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Gruppo in fibra SPME carbossene/polidimetilsilossano (CAR/PDMS), d_f 85 μm(CAR/PDMS, needle size 24 ga, StableFlex, for use with autosampler

57319

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Gruppo in fibra SPME carbossene/polidimetilsilossano (CAR/PDMS), d_f 75 μm(CAR/PDMS, needle size 24 ga, for use with autosampler

24246

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Carboxen^®-1010 PLOT Capillary GC Column, L × I.D. 30 m × 0.32 mm, average thickness 15 μm

57334-U

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Gruppo in fibra SPME carbossene/polidimetilsilossano (CAR/PDMS), d_f 85 μm(CAR/PDMS, needle size 24 ga, for use with manual holder, StableFlex fiber

57343-U

Supelco

Gruppo in fibra SPME carbossene/polidimetilsilossano (CAR/PDMS), d_f 75 μm(CAR/PDMS, needle size 23 ga, for use with autosampler

57344-U

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Gruppo in fibra SPME carbossene/polidimetilsilossano (CAR/PDMS), d_f 75 μm(CAR/PDMS, needle size 23 ga, for use with manual holder

24241-U

Supelco

Colonna capillare per GC Carboxen^®-1006 PLOT, L × I.D. 30 m × 0.32 mm, average thickness 15 μm

11072-U

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Adsorbente Carboxen^®, matrix Carboxen^® 572, 20-45 mesh, bottle of 10 g

28718-U

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Tenax^® TA / Carboxen^® 1018, glass TD tube, preconditioned, O.D. × L 1/4 in. × 3 1/2 in., Sealed with (Swagelok^® End-Fittings), pkg of 10 ea

57914-U

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Gruppo in fibra SPME divinilbenzene/carbossene/polidimetilsilossano (DVB/CAR/PDMS), for use with autosampler, needle size 23 ga, metal alloy fiber, fiber L 2 cm

54041-U

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Carboxen^® 1000 Reversible SPE Tube, 200 mg/mL, pk 30

10477-U

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Adsorbente Carboxen^®, matrix Carboxen^® 1000, 40-60 mesh, bottle of 50 g

10471

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Adsorbente Carboxen^®, matrix Carboxen^® 1003, 40-60 mesh, bottle of 10 g

10269

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Adsorbente Carboxen^®, matrix Carboxen^® 569, 20-45 mesh, bottle of 10 g

23446-U

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ORBO^™-93 Sorbent Tube, ORBO tube I.D. × L 6 mm × 95 mm, Bed A 180 mg, Bed B 90 mg, 60/80 mesh, pkg of 25 ea

504831

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SPME Portable Field Sampler, coating CAR/PDMS

57912-U

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Gruppo in fibra SPME divinilbenzene/carbossene/polidimetilsilossano (DVB/CAR/PDMS), for use with autosampler, needle size 23 ga, metal alloy fiber, fiber L 1 cm

11048-U

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Adsorbente Carboxen^®, matrix Carboxen^® 569, 20-45 mesh, bottle of 500 g