Yield effects on 2-methoxy-3-isobutylpyrazine concentration in cabernet sauvignon using a solid phase microextraction gas chromatography/mass spectrometry method.

Yield effects on 2-methoxy-3-isobutylpyrazine concentration in cabernet sauvignon using a solid phase microextraction gas chromatography/mass spectrometry method.

Journal of agricultural and food chemistry (2004-08-19)

Dawn M Chapman, John H Thorngate, Mark A Matthews, Jean-Xavier Guinard, Susan E Ebeler

ABSTRACT

A rapid and automated solid phase microextraction (SPME) stable isotope dilution gas chromatography/mass spectrometry (GC-MS) method for 2-methoxy-3-isobutylpyrazine (MIBP) quantification in red wine was developed. Wines with 30% (w/v) NaCl and 2-methoxy-(2)H(3)-3-isobutylpyrazine internal standard were sampled with a 2 cm divinylbenzene/carboxen/poly(dimethylsiloxane) SPME fiber for 30 min at 40 degrees C and analyzed by GC-MS. The method was used to measure MIBP concentrations in Cabernet Sauvignon wines that were produced from six winter pruning treatments over two vintages. MIBP concentrations were significantly negatively correlated with buds per vine. In addition, the MIBP concentration was directly related to sensory vegetal intensity ratings obtained by descriptive analysis.

MATERIALI

N° Catalogo

Marchio

Descrizione del prodotto

57348-U

Supelco

Gruppo in fibra SPME divinilbenzene/carbossene/polidimetilsilossano (DVB/CAR/PDMS), needle size 24 ga, StableFlex

57328-U

Supelco

Gruppo in fibra SPME divinilbenzene/carbossene/polidimetilsilossano (DVB/CAR/PDMS), needle size 24 ga, for use with manual holder

57298-U

Supelco

Gruppo in fibra SPME divinilbenzene/carbossene/polidimetilsilossano (DVB/CAR/PDMS), needle size 23 ga, StableFlex, for use with autosampler

57299-U

Supelco

Gruppo in fibra SPME divinilbenzene/carbossene/polidimetilsilossano (DVB/CAR/PDMS), needle size 23 ga, StableFlex, for use with manual holder or autosampler, fiber L 2 cm

57329-U

Supelco

Gruppo in fibra SPME divinilbenzene/carbossene/polidimetilsilossano (DVB/CAR/PDMS), needle size 24 ga, StableFlex, for use with autosampler

20358

Supelco

ORBO^™ 90 Carboxen^® 564 (20/45), 160/80 mg, W,F,F separators, O.D. × L 6 mm × 75 mm, pkg of 25 ea, for analyte group MEK (methylethyl ketone)

20355

Supelco

ORBO^™ 78 HBr on Carboxen^® 564 specially cleaned (20/45), 400/200 mg, W,W,W separators, O.D. × L 6 mm × 110 mm, pkg of 25 ea

25467

Supelco

Carboxen^®-1010 PLOT Capillary GC Column, L × I.D. 30 m × 0.53 mm, average thickness 30 μm

57318

Supelco

Gruppo in fibra SPME carbossene/polidimetilsilossano (CAR/PDMS), d_f 75 μm(CAR/PDMS, for use with manual holder, needle size 24 ga

10478-U

Supelco

Adsorbente Carboxen^®, matrix Carboxen^® 1000, 60-80 mesh, bottle of 10 g

25461

Supelco

Colonna capillare per GC Carboxen^®-1006 PLOT, L × I.D. 30 m × 0.53 mm, average thickness 30 μm

57335-U

Supelco

Gruppo in fibra SPME carbossene/polidimetilsilossano (CAR/PDMS), d_f 85 μm(CAR/PDMS, needle size 24 ga, StableFlex, for use with autosampler

57295-U

Supelco

Gruppo in fibra SPME carbossene/polidimetilsilossano (CAR/PDMS), d_f 85 μm(CAR/PDMS, needle size 23 ga, StableFlex, for use with autosampler

57319

Supelco

Gruppo in fibra SPME carbossene/polidimetilsilossano (CAR/PDMS), d_f 75 μm(CAR/PDMS, needle size 24 ga, for use with autosampler

24246

Supelco

Carboxen^®-1010 PLOT Capillary GC Column, L × I.D. 30 m × 0.32 mm, average thickness 15 μm

W313203

Sigma-Aldrich

2-Isobutyl-3-methoxypyrazine, ≥99%, FG

57334-U

Supelco

Gruppo in fibra SPME carbossene/polidimetilsilossano (CAR/PDMS), d_f 85 μm(CAR/PDMS, needle size 24 ga, for use with manual holder, StableFlex fiber

57343-U

Supelco

Gruppo in fibra SPME carbossene/polidimetilsilossano (CAR/PDMS), d_f 75 μm(CAR/PDMS, needle size 23 ga, for use with autosampler

47528-U

Supelco

2-Isobutyl-3-methoxypyrazine solution, certified reference material

297666

Sigma-Aldrich

2-Isobutyl-3-methoxypyrazine, 99%

57344-U

Supelco

Gruppo in fibra SPME carbossene/polidimetilsilossano (CAR/PDMS), d_f 75 μm(CAR/PDMS, needle size 23 ga, for use with manual holder

24241-U

Supelco

Colonna capillare per GC Carboxen^®-1006 PLOT, L × I.D. 30 m × 0.32 mm, average thickness 15 μm

54041-U

Supelco

Carboxen^® 1000 Reversible SPE Tube, 200 mg/mL, pk 30

11072-U

Supelco

Adsorbente Carboxen^®, matrix Carboxen^® 572, 20-45 mesh, bottle of 10 g

10477-U

Supelco

Adsorbente Carboxen^®, matrix Carboxen^® 1000, 40-60 mesh, bottle of 50 g

57914-U

Supelco

Gruppo in fibra SPME divinilbenzene/carbossene/polidimetilsilossano (DVB/CAR/PDMS), for use with autosampler, needle size 23 ga, metal alloy fiber, fiber L 2 cm

28718-U

Supelco

Tenax^® TA / Carboxen^® 1018, glass TD tube, preconditioned, O.D. × L 1/4 in. × 3 1/2 in., Sealed with (Swagelok^® End-Fittings), pkg of 10 ea

10269

Supelco

Adsorbente Carboxen^®, matrix Carboxen^® 569, 20-45 mesh, bottle of 10 g

10471

Supelco

Adsorbente Carboxen^®, matrix Carboxen^® 1003, 40-60 mesh, bottle of 10 g

23446-U

Supelco

ORBO^™-93 Sorbent Tube, ORBO tube I.D. × L 6 mm × 95 mm, Bed A 180 mg, Bed B 90 mg, 60/80 mesh, pkg of 25 ea