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Key Documents

814005

TSKgel® Octadecyl-NPR (2.5 µm) HPLC Columns

L x I.D. 3.5 cm × 4.6 mm, C18 (octadecyl) phase

Sinonimo/i:

TSKgel® Reversed Phase HPLC Column (Polymer-based)

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About This Item

Codice UNSPSC:
41115700

product name

TSKgel® Octadecyl-NPR HPLC Column, phase C18 (octadecyl), L × I.D. 3.5 cm × 4.6 mm, 2.5 μm particle size

Materiali

stainless steel

Nome Commerciale

TSKgel®

Produttore/marchio commerciale

Tosoh 14005

Grado di funzionalizzazione

1 meq/mL ligand density (estimate)

Parametri

≤0.5 M salt concentration
0-100% organic solvent
0-60 °C temperature
1.6 mL/min flow rate
200 bar max. pressure

tecniche

HPLC: suitable

Lungh. × D.I.

3.5 cm × 4.6 mm

Matrice

polymer particle platform

Gruppo funzionale matrice

C18 (octadecyl) phase

Dimensione particelle

2.5 μm

pH di lavoro

2-12

Tecnica di separazione

reversed phase

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Descrizione generale

Highly cross-linked polymethacrylate base material of TSKgel Octadecyl-NPR provides excellent stability in high pH buffer systems and can withstand rigorous cleaning with either acid or base. NPR, non-porous resin, columns are prepared from non-porous methacrylate particles of uniform 2.5 micron size, which provides high efficiency separations and fast anaylses of peptides and proteins. The non-porous particle structure limits product isolation to sub-microgram loads.
Polymer-based particles for reversed-phase (RP) HPLC generally have two benefits over silica-based particles: They are more tolerant of high and low pH operating conditions and they do not have the silanol interactions that cause peak tailing of some basic analytes. Also, some silicas have an intrinsic metal content that may cause chelation problems with sensitive biomolecules, leading to poor recovery. Polymeric TSKgel columns come in C18 and phenyl functional groups and a range of particle porosities. Fast kinetics and quantitative protein recovery at submicrogram loading are characteristic of nonporous resin-based TSKgel Octadecyl-NPR columns.
  • Octadecyl-2PW particles have 125Å pores
  • Octadecyl-4PW particles have 500Å pores
  • Phenyl-5PW RP particles have 1000Å pores
  • Octadecyl-NPR particles are nonporous

Stato fisico

Shipped in 70% methanol - 30% waters (v/v).

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Note legali

TSKgel is a registered trademark of Tosoh Corporation

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