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50994-U

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BIOshell Glycan (2.7 μm) HPLC Columns

L × I.D. 15 cm × 2.1 mm, HPLC Column

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About This Item

Codice UNSPSC:
41115700
NACRES:
SB.52

product name

BIOshell Glycan HPLC Columns, L × I.D. 15 cm × 2.1 mm, 2.7 μm particle size

Materiali

stainless steel hardware (column)

Livello qualitativo

agenzia

suitable for USP L86

Descrizione

shell thickness (0.5 μm)
solid core (1.7 μm)

Nome Commerciale

BIOshell

Caratteristiche

endcapped: no

Classi chimiche degli analiti

glycans

Produttore/marchio commerciale

BIOshell

Confezionamento

1 ea of

Parametri

1000 bar max. pressure (14500 psi)
1000 bar pressure (14500 psi)
60 °C max. temp.

tecniche

HPLC: suitable
LC/MS: suitable
UHPLC-MS: suitable
UHPLC: suitable

Lungh. × D.I.

15 cm × 2.1 mm

Area superficiale

135 m2/g

Matrice

spherical silica particle platform
superficially porous particle

Gruppo funzionale matrice

hydroxy, penta- phase

Dimensione particelle

2.7 μm

Dimensione pori

90 Å

operating pH range

2-9

Tecnica di separazione

hydrophilic interaction (HILIC)

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Descrizione generale

BIOshell Glycan HPLC Columns are specifically engineered to deliver fast, high resolution, reproducible glycan identification using HILIC chromatography. The Fused-Core columns provide improved resolution when compared to conventional LC methods thus allowing more reliable quantitation and confident identification of glycans.

View the BIOshell Glycan HPLC Columns Flyer

Applicazioni

BIOshell Glycan HPLC Columns are high-performance liquid chromatography columns with Fused-Core particle design. Their column chemistry is suitable for hydrophilic interactive liquid chromatography (HILIC) analysis of biomolecules like oligosaccharides.

Caratteristiche e vantaggi

BIOshell Glycan HPLC Columns are compatible both with UHPLC and HPLC systems. They are specifically engineered for the fast and high-resolution seperation of biomolecules. These columns operate at high flow rates and have 40% higher efficiency than fully porous particles of the same size. They are stable at high temperatures, which increases the throughput and enhances the peak shape.

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Articoli

The BIOshell™ Glycan column is ideal for the separation and quantitation of isolated glycans using the HILIC method for accurate glycoprotein analysis.

The use of PNGase Fast denaturing buffer and enzyme yielded results similar to a conventional 20-hour protocol with overnight digest while reducing workflow time to about 1 hour with a 15-minute digest.

Step-by-step workflows for the intact mass analysis, peptide mapping, and N-glycan analysis of the monoclonal antibody― adalimumab, for an accurate characterization of the critical quality attributes (CQAs) to ensure drug safety and efficacy. Read more.

Protocolli

Contenuto correlato

HPLC separates and identifies large biomolecules like proteins and peptides by pumping the sample through a sorbent-packed column.

Il team dei nostri ricercatori vanta grande esperienza in tutte le aree della ricerca quali Life Science, scienza dei materiali, sintesi chimica, cromatografia, discipline analitiche, ecc..

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