ZRB1592

Anti-EEA1 Antibody, clone 1H18 ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• Codice UNSPSC: 12352203
• NACRES: NA.41

Proprietà

• Origine biologica: rabbit
• Livello qualitativo: 200
• Ricombinante: expressed in HEK 293 cells
• Coniugato: unconjugated
• Forma dell’anticorpo: purified antibody
• Tipo di anticorpo: primary antibodies
• Clone: 1H18, recombinant monoclonal
• Descrizione: recombinant, expressed in HEK 293 cells
• Nome Commerciale: ZooMAb^® learn more
• Forma fisica: lyophilized
• PM: calculated mol wt 162.47 kDa; observed mol wt ~160 kDa
• Purificato mediante: using Protein A
• Reattività contro le specie: rat, human
• Reattività contro le specie (prevista in base all’omologia): monkey
• Confezionamento: antibody small pack of 25 μL
• Caratteristiche più verdi: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• Convalida avanzata: recombinant expression; Learn more about Antibody Enhanced Validation
• sustainability: Greener Alternative Product
• tecniche: affinity binding assay: suitable; immunocytochemistry: suitable; immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable; western blot: suitable
• Isotipo: IgG
• Sequenza dell’epitopo: N-terminal half
• N° accesso ID proteina: NP_003557
• N° accesso UniProt: Q15075
• Categoria alternativa più verde: Aligned,
• Condizioni di spedizione: ambient
• Temperatura di conservazione: 2-8°C
• modifica post-traduzionali bersaglio: unmodified

Descrizione

Descrizione generale

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

Specificità

Clone 1H18 is a ZooMAb^® Rabbit recombinant monoclonal antibody that specifically detects Early endosome antigen 1 (EEA1). It targets an epitope within 24 amino acids from the N-terminal half.

Immunogeno

KLH-conjugated linear peptide corresponding to 24 amino acids from the N-terminal half of human Early endosome antigen 1 (EEA1).

Applicazioni

Quality Control Testing

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: A 1:10,000 dilution of this antibody detected Early endosome antigen 1 (EEA1) in HeLa cell lysate.

Tested applications

Western Blotting Analysis: A 1:10,000 dilution from a representative lot detected Early endosome antigen 1 (EEA1) in PC12, SHSY5Y, and HepG2 cell lysates.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Early endosome antigen 1 (EEA1) in A431, HeLa and HepG2 cells.s.

Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected Early endosome antigen 1 (EEA1) in human cerebral cortex tissue sections.

Affinity Binding Assay: A representative lot of this antibody bound Early endosome antigen 1 (EEA1) with a KD of 1 x 10-12 in an affinity binding assay.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Anti-EEA1, clone 1H18 ZooMAb^®, Cat. No. ZRB1592, is a recombinant Rabbit monoclonal antibody that detects Early endosome antigen 1 (EEA1) and used in Affinity Binding Assay, Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.

Descrizione del bersaglio

Early endosome antigen 1 (UniProt: Q15075; also known as Endosome-associated protein p162, Zinc finger FYVE domain-containing protein 2, EEA1) is encoded by the EEA1 (also known as ZFYVE2) gene (Gene ID: 8411) in human. EEA1 is a homodimeric peripheral membrane protein that binds to phospholipid vesicles containing phosphatidylinositol 3-phosphate and participates in endosomal trafficking. It binds to Rab5 proteins that have been activated by GTP-binding. The stable association of EEA1 with the endosomal membrane is regulated by PI3 kinase, Rab5 and Ca2+/calmodulin. At its carboxy terminal, it contains a PYVE type zinc finger domain (aa 1352-1410) that mediates its interaction with phosphatidylinositol 3-phosphate in membranes of early endosomes and penetrates bilayers. The FYVE domain insertion into phosphatidylinositol 3-phosphate-enriched membranes is shown to substantially increase in acidic conditions. When endosome fusion is not required, EEA1 serves as a substrate for p97, promoting EEA1 dissociation and endosome independence. This ZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.

Stato fisico

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb^® formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

Ricostituzione

300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Stoccaggio e stabilità

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Note legali

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 11 - Combustible Solids
• Classe di pericolosità dell'acqua (WGK): WGK 1
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable