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Key Documents

ZMS1042

Sigma-Aldrich

Anti-ssDNA Antibody, clone 16-19 ZooMAb® Mouse Monoclonal

enhanced validation
greener alternative

recombinant, expressed in HEK 293 cells

Sinonimo/i:

Single stranded DNA

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About This Item

Codice UNSPSC:
12352203
NACRES:
NA.43

Origine biologica

mouse

Livello qualitativo

Ricombinante

expressed in HEK 293 cells

Coniugato

unconjugated

Forma dell’anticorpo

purified antibody

Tipo di anticorpo

primary antibodies

Clone

16-19, recombinant monoclonal

Nome Commerciale

ZooMAb® learn more

Forma fisica

lyophilized

Purificato mediante

using protein G

Reattività contro le specie

bovine

Reattività contro le specie (prevista in base all’omologia)

all

Confezionamento

antibody small pack of 25 μL

Caratteristiche più verdi

Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.

Convalida avanzata

recombinant expression
Learn more about Antibody Enhanced Validation

sustainability

Greener Alternative Product

tecniche

ELISA: suitable
dot blot: suitable
immunocytochemistry: suitable

Categoria alternativa più verde

Condizioni di spedizione

ambient

Temperatura di conservazione

2-8°C

modifica post-traduzionali bersaglio

unmodified

Descrizione generale

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.

Specificità

Clone 16-19 is a ZooMAb® Mouse recombinant monoclonal antibody that specifically detects ssDNA.

Immunogeno

Hybridoma generated by fusion of splenocytes from non-immunized BW and myeloma cell line Sp 2/0-Ag 14.

Applicazioni

Quality Control Testing

Evaluated by Dot Blot with ssDNA from Calf thymus.

Dot Blot Analysis: A 1:1,000 dilution of this antibody detected ssDNA from Calf thymus.

Tested applications

Enzyme Immunoassay Analysis (ELISA): Serial of dilutions from a representative lot detected ssDNA from Calf thymus.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected ssDNA in Molt 4 cells treated with Etoposide (10 M; overnight).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Evaluated by Dot Blot with ssDNA from Calf thymus.

Dot Blot Analysis: A 1:1,000 dilution of this antibody detected ssDNA from Calf thymus.

Descrizione del bersaglio

DNA, the carrier of genetic information, is a crucial part of basic biological research and synthetic biology. It exists as a double-helix structure and the two DNA strands known as polypeptides are composed of monomeric units known as nucleotides. Each nucleotide consists of four bases: adenine, guanine, cytosine, thymine and a deoxyribose sugar moiety. The nitrogenous bases of the two separate strands are bound together by base pairing (A-T and C-G) to create a double stranded DNA. Under certain conditions, these two strands can come apart (melting) to generate two single-stranded DNA. Single-stranded DNA (ssDNA) is a crucial DNA damage signal found at the sites of DNA damage that helps in recruitment of checkpoint kinases to the site of damage. This leads to cell cycle arrest, transcriptional upregulation of DNA repair genes, and modulation of DNA replication pathways. Hence, ssDNA is considered as a key intermediate in nearly all biochemical reactions involving DNA replication and DNA repair. However, the ssDNA structure is not stable and single-stranded DNA-binding proteins (SSB) hold the ssDNA intact and exposed during the course of its function, such as DNA transcription, recombination, and repair, and serve as template for opposite strand DNA synthesis. Antibodies against ssDNA are commonly used in ELISA-based test for the quantitative determination of IgG antibodies against ssDNA and have been used in serological detection of systemic lupus erythematosus (SLE) and drug induced lupus. This ZooMAbZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Rekvig, OP. (2022). Front. Immunol. 12; 808008; Bantele, SCS., et al. (2019). Nat. Commun. 10; Article 944; Ballard, DW., and Voss EW. (1985). J. Immunol. 135(5); 3372-3380).

Stato fisico

Purified recombinant mouse monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

Ricostituzione

300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Stoccaggio e stabilità

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Note legali

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Codice della classe di stoccaggio

11 - Combustible Solids

Classe di pericolosità dell'acqua (WGK)

WGK 1

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable


Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

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