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Y1751

Sigma-Aldrich

Yeast Synthetic Drop-out Medium Supplements

without histidine

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About This Item

Numero MDL:
MFCD01095679
Codice UNSPSC:
12000000
NACRES:
ND.02

Livello qualitativo

100

Forma fisica

powder

applicazioni

food and beverages

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Applicazioni

The selection of plasmids in yeast is based on the use of auxotrophic mutant strains that cannot grow without a specific media component (an amino acid, purine or pyrimidine). Transformation with a plasmid containing the mutated gene enables the transformant to grow on a medium lacking the required component. Sigma′s Yeast Synthetic Drop-Out Media Supplements create a richer medium for better yield and growth rate, and increase the probability of successful transformations when screening libraries or performing gene knock-outs.

Componenti

The supplements contain all of the following components except as indicated:
Amino acids: All standard amino acids except for histidine. All the amino acids are present at a concentration of 76 mg/lL except for leucine, which is present at 380 mg/L.
Other nutrients: Adenine (18 mg/L), inositol (76 mg/L), uracil (76 mg/L), p-aminobenzoic acid (8 mg/L).

Altre note

Mixtures of amino acids and other nutrients to be added to Yeast Nitrogen Base Without Amino Acids.

Quantità

1.92 g will supplement 1 liter of medium.

Codice della classe di stoccaggio

13 - Non Combustible Solids

Classe di pericolosità dell'acqua (WGK)

WGK 1

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Dispositivi di protezione individuale

Eyeshields, Gloves, type N95 (US)

Certificati d'analisi (COA)

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Tamara Suhm et al.
Cell metabolism, 27(6), 1309-1322 (2018-05-15)
Cellular proteostasis is maintained via the coordinated synthesis, maintenance, and breakdown of proteins in the cytosol and organelles. While biogenesis of the mitochondrial membrane complexes that execute oxidative phosphorylation depends on cytoplasmic translation, it is unknown how translation within mitochondria impacts

Articoli

Introduction to Yeast Media

Technical Article on yeast media. Yeasts are eukaryotic microorganisms whose genomes have been comprehensively studied and some have been sequenced.

Protein Expression Systems

The development of genetic engineering and cloning has opened many possibilities of expression and isolation of heterologous proteins for research purposes. Considerable advances in technology have enabled expression and isolation of recombinant proteins in large scale.

Protocolli

Yeast Growth Protocols

Yeasts are considered model systems for eukaryotic studies as they exhibit fast growth and have dispersed cells. Yeast cultures can be grown, maintained, and stored in liquid media or on agar plates using techniques similar to those for bacterial cultures.

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