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XNAB2

Sigma-Aldrich

Extract-N-Amp™ Blood PCR Kit

sufficient for 100 extractions, sufficient for 100 amplifications

Sinonimo/i:

Blood direct PCR kit

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About This Item

Codice UNSPSC:
41106303
NACRES:
NA.55

impiego

sufficient for 100 amplifications
 sufficient for 100 extractions
 sufficient for 100 reactions

Livello qualitativo

200

Caratteristiche

dNTPs included
hotstart

tecniche

PCR: suitable

Colore

colorless

Condizioni di spedizione

wet ice

Temperatura di conservazione

−20°C

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Descrizione generale

The Extract-N-Amp™ Blood PCR Kits contain all of the reagents necessary to rapidly extract host genomic DNA from whole blood and amplify targets of interest by direct PCR. This novel extraction system eliminates the need for any type of purification, organic extraction, centrifugation, heating, filtration or alcohol precipitation. The kit also includes a PCR ReadyMix™, specially formulated for amplification directly from the extract. This formulation uses an antibody based hot start, for specific amplification.

Applicazioni

Extract-N-Amp™ Blood PCR Kit has been used to extract DNA from genomes and dried blood spot samples. It has also been used for polymerase chain reaction (PCR).

Caratteristiche e vantaggi

  • Efficient 8 minute prep allows greater speed and throughput
  • No need for any type of purification, organic extraction, centrifugation or alcohol precipitation
  • Simple, 3 step procedure with no special equipment required
  • Hot start antibody included for highly specific PCR amplification of genomic DNA
  • Compatible with multiple format (single tube or 96-well plate)
  • Can be used with whole blood or blood cards
  • Extract stable at 4 °C for at least 6 months

Principio

Genomic DNA is extracted from 10 μl of whole blood by simply adding the Extraction Solution and incubating for 5 minutes at room temperature. The Neutralization Solution is added to the extract to counteract inhibitory substances prior to PCR. A portion of the DNA extract is then added to the specially formulated PCR ReadyMix.

Altre note

For additional information, please see www.sigma-aldrich.com/extract-n-amp.

Note legali

Purchase of this product includes an immunity from suit under patents specified in the product insert to use only the amount purchased for the purchaser′s own internal research. No other patent rights (such as 5′ Nuclease Process patent rights) are conveyed expressly, by implication, or by estoppel. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.
Antibody licensed for in vitro research use under U.S. Patent No. 5,338,671 and 5,587,287, and corresponding patents in other countries.
Extract-N-Amp is a trademark of Sigma-Aldrich Co. LLC
ReadyMix is a trademark of Sigma-Aldrich Co. LLC

I componenti del kit sono disponibili anche separatamente

N° Catalogo
Descrizione
SDS
  • L3289Lysis Solution for BloodSDS
  • N9784Neutralization Solution for BloodSDS
  • P8115Extract-N-Amp™ PCR ReadyMix™ for BloodSDS

Prodotti correlati

N° Catalogo
Descrizione
Determinazione del prezzo

XNAB

REDExtract-N-Amp™ Blood PCR Kit, sufficient for 100 extractions, sufficient for 100 amplifications

XNABR

REDExtract-N-Amp™ Blood PCR Kit, sufficient for 1000 extractions, sufficient for 1000 amplifications

XNAB2RE

Extract-N-Amp™ Blood PCR Kit, sufficient for 1000 extractions, sufficient for 5000 amplifications

XNABS

REDExtract-N-Amp™ Blood PCR Kit, sufficient for 10 extractions, sufficient for 10 amplifications

P8240

REDExtract-N-Amp™ PCR ReadyMix™ for Blood, 12 mL sufficient for 1000 amplifications

P8115

Extract-N-Amp™ PCR ReadyMix™ for Blood, 12 mL sufficient for 1000 amplifications

L3289

LYSIS SOLUTION FOR BLOOD, sufficient for 100 reactions, for molecular biology

N9784

Neutralization Solution for Blood, sufficient for 100 reactions, sufficient for 1000 reactions, for molecular biology

Pittogrammi

Corrosion
GHS05

Avvertenze

Danger

Indicazioni di pericolo

H290,H314

Classi di pericolo

Eye Dam. 1 - Met. Corr. 1 - Skin Corr. 1B

Codice della classe di stoccaggio

8A - Combustible corrosive hazardous materials

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

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Min Lu et al.
Blood, 120(15), 3098-3105 (2012-08-09)
Interferon (IFN-α) is effective therapy for polycythemia vera (PV) patients, but it is frequently interrupted because of adverse events. To permit the long-term use of IFN, we propose combining low doses of IFN with Nutlin-3, an antagonist of MDM2, which
Roger G Rank et al.
Infection and immunity, 77(3), 1216-1221 (2009-01-14)
Over the last several years, four different phages of chlamydiae, in addition to a phage associated with Chlamydia psittaci isolated from an ornithosis infection in ducks over 25 years ago, have been described and characterized. While these phages and their
Bo G Winkel et al.
BMC medical genetics, 12, 22-22 (2011-02-11)
The use of dried blood spots (DBS) samples in genomic workup has been limited by the relative low amounts of genomic DNA (gDNA) they contain. It remains to be proven that whole genome amplified DNA (wgaDNA) from stored DBS samples
Mads Vilhelm Hollegaard et al.
Molecular genetics and metabolism, 108(4), 225-231 (2013-02-21)
DNA methylation is the most common DNA modification and perhaps the best described epigenetic modification. It is believed to be important for genomic imprinting and gene regulation and has been associated with the development of diseases such as schizophrenia and
Maria C Walsh et al.
PloS one, 6(11), e27177-e27177 (2011-12-02)
We assessed the effect of short-term feeding of genetically modified (GM: Bt MON810) maize on immune responses and growth in weanling pigs and determined the fate of the transgenic DNA and protein in-vivo. Pigs were fed a diet containing 38.9%
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Articoli

Extract-N-Amp™ Blood PCR Kit Protocol

The Extract-N-Amp Blood PCR Kits contain all the reagents needed to rapidly extract and amplify human genomic DNA from whole blood, whole blood dried on a blood card, and cultured mammalian cells.

Extract-N-Amp Reagent Provides a Highly Effective Way of Generating PCR Products from FTA® Blood Cards

Extract-N-Amp is a versatile, combined DNA extraction and amplification kit intended to simplify the generation of PCR products from a range of sample types. This methodology can be used without modification to perform PCR on blood samples stored on FTA blood cards (Whatman) which removes the need for the laborious and costly washing procedures that the standard FTA protocol stipulates. This technique removes the variability between sample preparations and maximizes the number of PCR reactions that can be performed on individual FTA blood card samples.

Genomic DNA Sample Purification and Quality Assessment

The availability of simple methods for purification of DNA and RNA has greatly facilitated the analysis and characterization of the genome and gene expression. There is a demand to isolate DNA and RNA rapidly and conveniently from a variety of cellular sources, including cells and tissues from mammalian, plant and bacterial cultures.

Il team dei nostri ricercatori vanta grande esperienza in tutte le aree della ricerca quali Life Science, scienza dei materiali, sintesi chimica, cromatografia, discipline analitiche, ecc..

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