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XNAS2

Sigma-Aldrich

Extract-N-Amp Seed PCR Kit

sufficient for 100 extractions, sufficient for 100 amplifications

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About This Item

Codice UNSPSC:
41106303
NACRES:
NA.55

impiego

sufficient for 100 amplifications
sufficient for 100 extractions
sufficient for 100 reactions

Caratteristiche

dNTPs included
hotstart

tecniche

PCR: suitable

applicazioni

agriculture

Condizioni di spedizione

dry ice

Temperatura di conservazione

−20°C

Descrizione generale

The Extract-N-Amp Seed PCR Kit provides all reagents necessary to rapidly extract DNA from a wide variety of plant seeds and amplify targets of interest by direct PCR. A novel extraction method eliminates the need for long enzymatic digestions. The kit also includes a specially formulated hot start PCR reaction mix for amplification directly from the extract.

Applicazioni

Extract-N-Amp seed PCR Kit has been used for DNA extraction. It has aslo been used for extraction and amplification of DNA from symptomatic wheat seeds.

Caratteristiche e vantaggi

  • Perfect for quick genotyping results
  • Rapid extraction of genomic DNA for PCR in 15 minutes
  • No long enzymatic digestions
  • Hot Start antibody for highly specific PCR amplification of genomic DNA
  • Compatible with a wide variety of seed sources
  • Extracts are stable at 4 °C for atleast 6 months, allowing for multiple reassays
  • Phenol/chloroform extraction or alcohol precipitation not required
  • Column purification or centrifugation not required

Altre note

For additional information, please see www.sigma-aldrich.com/extract-n-amp.

Note legali

Purchase of this product includes an immunity from suit under patents specified in the product insert to use only the amount purchased for the purchaser′s own internal research. No other patent rights (such as 5′ Nuclease Process patent rights) are conveyed expressly, by implication, or by estoppel. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.
Antibody licensed for in vitro research use under U.S. Patent No. 5,338,671 and 5,587,287, and corresponding patents in other countries.

Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: 5,789,224, 5,618,711, 6,127,155 and claims outside the US corresponding to expired US Patent No. 5,079,352. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser′s own internal research. No right under any other patent claim, no right to perform any patented method, and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser′s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patents require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.
Extract-N-Amp is a trademark of Sigma-Aldrich Co. LLC

Pittogrammi

Health hazardEnvironment

Avvertenze

Danger

Indicazioni di pericolo

Classi di pericolo

Aquatic Chronic 2 - Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - Skin Sens. 1

Codice della classe di stoccaggio

10 - Combustible liquids

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable


Certificati d'analisi (COA)

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Brigitte Marazzi et al.
American journal of botany, 93(2), 288-303 (2006-02-01)
Senna (Leguminosae) is a large, widespread genus that includes species with enantiostylous, asymmetric flowers and species with extrafloral nectaries. Clarification of phylogenetic relationships within Senna based on parsimony analyses of three chloroplast regions (rpS16, rpL16, and matK) provides new insights
Helen L Rose et al.
PloS one, 6(7), e22668-e22668 (2011-08-06)
Eight DNA extraction products or methods (Applied Biosystems PrepFiler Forensic DNA Extraction Kit; Bio-Rad Instagene Only, Bio-Rad Instagene & Spin Column Purification; EpiCentre MasterPure DNA & RNA Kit; FujiFilm QuickGene Mini80; Idaho Technologies 1-2-3 Q-Flow Kit; MoBio UltraClean Microbial DNA

Articoli

The availability of simple methods for purification of DNA and RNA has greatly facilitated the analysis and characterization of the genome and gene expression. There is a demand to isolate DNA and RNA rapidly and conveniently from a variety of cellular sources, including cells and tissues from mammalian, plant and bacterial cultures.

Il team dei nostri ricercatori vanta grande esperienza in tutte le aree della ricerca quali Life Science, scienza dei materiali, sintesi chimica, cromatografia, discipline analitiche, ecc..

Contatta l'Assistenza Tecnica.