UC9601
UltraClear™ Sequencing Reaction Clean-Up Kit
pkg of 1 plates, sufficient for 1 96-well plate purifications
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About This Item
Codice UNSPSC:
41105600
NACRES:
NA.52
Prodotti consigliati
impiego
sufficient for 1 96-well plate purifications
Livello qualitativo
Confezionamento
pkg of 1 plates
Temperatura di conservazione
15-25°C
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Descrizione generale
The UltraClear Sequencing Reaction Clean-Up Plates offer a rapid and simple method for the clean-up of DNA sequencing reactions in a 96-well format. The plates use ultrafiltration membranes to separate low molecular weight contaminants, such as unincorporated dye terminators, dNTPs, and residual salts from the sequencing reaction products.
Following thermocycling, the sequencing reactions are diluted with Sequencing Solution. This mixture is then filtered through the UltraClear plate by centrifugation. The sequencing reaction products are retained on the surface of the ultrafiltration membrane while the lower molecular weight contaminants pass through the membrane and are collected as waste. The purified sequencing products are then resuspended in Sequencing Solution and are ready for injection onto capillary-based DNA analyzers.
Following thermocycling, the sequencing reactions are diluted with Sequencing Solution. This mixture is then filtered through the UltraClear plate by centrifugation. The sequencing reaction products are retained on the surface of the ultrafiltration membrane while the lower molecular weight contaminants pass through the membrane and are collected as waste. The purified sequencing products are then resuspended in Sequencing Solution and are ready for injection onto capillary-based DNA analyzers.
Caratteristiche e vantaggi
- Effective clean-up of ABI BigDye® chemistries
- Simple centrifugation-based procedure
- Convenient 96-well format
Altre note
For additional information, please see www.sigma-aldrich.com/sequencing.
Note legali
BigDye is a registered trademark of Applera Corporation or its subsidiaries in the US and/or certain other countries
UltraClear is a trademark of Sigma-Aldrich Co. LLC
Codice della classe di stoccaggio
10 - Combustible liquids
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
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