T7572
Trombina
400-1000 NIH units/mg protein
Sinonimo/i:
Fattore IIa
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About This Item
Prodotti consigliati
Forma fisica
lyophilized powder
Livello qualitativo
Attività specifica
400-1000 NIH units/mg protein
Impurezze
HIV, hepatitis B and hepatitis C, tested negative
N° accesso UniProt
Temperatura di conservazione
−20°C
Informazioni sul gene
human ... F2(2147)
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Descrizione generale
Lyophilized powder containing sucrose, sodium chloride and Tris.
Thrombin is the final coagulation protease in regard to hemostasis, promoting both procoagulant and anticoagulant effects.
Applicazioni
Thrombin is used for site specific cleavage of recombinant fusion proteins containing an accessible thrombin recognition site for removal of affinity tags. Thrombin has been used in a study to assess an innovative flow cytometric approach for small-size platelet microparticles.
Azioni biochim/fisiol
Serin proteasi che scinde in modo selettivo i legami Arg-Gly nel fibrinogeno per formare fibrina e i fibrinopeptidi A e B.
Definizione di unità
Activity is expressed in NIH units obtained by direct comparison to a NIH Thrombin Reference Standard
Risultati analitici
The NIH assay procedure uses 0.2 ml diluted plasma (1:1 with saline) as a substrate and 0.1ml of thrombin sample (stabilized in a 1% buffered albumin solution) based on a modification of the method of Biggs. Only clotting times in the range of 15-25 seconds are used for determining thrombin concentrations.
Altre note
View more information on thrombin at www.sigma-aldrich.com/enzymeexplorer.
Esclusione di responsabilità
RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.
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Articoli
Thrombin Factor IIa is an endolytic serine protease that selectively cleaves the Arg--Gly bonds of fibrinogen to form fibrin and release fibrinopeptides A and B.
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