T5816
Tricina
BioPerformance Certified, suitable for cell culture, ≥99% (titration)
Sinonimo/i:
N-[tris(idrossimetil)metil]glicina
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About This Item
Formula condensata:
(HOCH2)3CNHCH2CO2H
Numero CAS:
Peso molecolare:
179.17
Beilstein:
1937804
Numero CE:
Numero MDL:
Codice UNSPSC:
12161700
ID PubChem:
NACRES:
NA.25
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Livello qualitativo
Grado
BioPerformance Certified
Saggio
≥99% (titration)
Forma fisica
crystalline powder
Condizioni di stoccaggio
dry at room temperature
tecniche
cell culture | mammalian: suitable
Impurezze
endotoxin and total aerobic microbial count, tested
Colore
white
Intervallo di pH utile
7.4-8.8
pKa (25 °C)
8.1
Punto di fusione
187 °C
Cationi in tracce
heavy metals (as Pb): ≤5 ppm
Assorbimento
≤0.02 at 290 at 20%
applicazioni
diagnostic assay manufacturing
general analytical
life science and biopharma
sample preparation
Stringa SMILE
OCC(CO)(CO)NCC(O)=O
InChI
1S/C6H13NO5/c8-2-6(3-9,4-10)7-1-5(11)12/h7-10H,1-4H2,(H,11,12)
SEQKRHFRPICQDD-UHFFFAOYSA-N
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Descrizione generale
Tricine is a zwitterionic biological buffer commonly used in enzyme assays, electrophoresis, and cell culture research. It has a higher negative charge compared to glycine, which enables it to migrate more rapidly. Tricine′s high ionic strength has the ability to cause more ion movement and less protein movement, resulting in the separation of low molecular weight proteins in lower percent acrylamide gels. It has been observed that Tricine is efficient in the separation of proteins ranging from 1 to 100 kDa through electrophoresis. According to a study, the 25 mmol/L Tricine buffer proved to be the most effective among ten other buffers tested for ATP assays using firefly luciferase. Further, Tricine is also used in capillary zone electrophoresis, high performance liquid chromatography and ion exchange chromatography.
Applicazioni
Tricine serves as a versatile buffering agent suitable for separating low molecular weight proteins in lower percent acrylamide gels, has demonstrated efficacy in ATP assays employing firefly luciferase, and has shown potential in scavenging hydroxyl radicals during the investigation of radiation-induced membrane damage. Additionally, it has played a role in isolating and purifying ribosomes engaged in the translation of specific mRNA and containing specific peptidyl-tRNA molecules. Tricine has also been employed in the synthesis of RNA oligonucleotides and the fabrication of high-density DNA microarrays, enabling the exploration of mechanisms underlying nucleic acid·ligand interactions and library preparation through photolithography.
Componente del tampone per la separazione dei peptidi a basso peso molecolare.
Caratteristiche e vantaggi
- Suitable as a Buffer component, for Electrophoresis and Protein separation
- Effective Buffering from pH 7.4-8.8 (25 °C) with a pKa of 8.1 (25 °C)
- Tested for Endotoxins and Total Aerobic Microbial Count
- Tested to confirm low levels of heavy metal contamination, ensuring suitability for various applications
Altre note
For additional information on our range of Biochemicals, please complete this form.
Prodotto comparabile
Codice della classe di stoccaggio
13 - Non Combustible Solids
Classe di pericolosità dell'acqua (WGK)
WGK 3
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
Dispositivi di protezione individuale
Eyeshields, Gloves, type N95 (US)
Certificati d'analisi (COA)
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Electrophoretic separations of proteins are widely used in proteomic analyses, and rely heavily on SDS electrophoresis. This mode of separation is almost exclusively used when a single dimension separation is performed, and generally represents the second dimension of two-dimensional separations.
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