T17917

Tricina

≥99% (titration), reagent grade, crystalline

• Sinonimo/i: N-[tris(idrossimetil)metil]glicina
• Formula condensata: (HOCH₂)₃CNHCH₂CO₂H
• Numero CAS: 5704-04-1
• Peso molecolare: 179.17
• Beilstein: 1937804
• Numero MDL: MFCD00004277
• Codice UNSPSC: 12161700
• NACRES: NA.25

Proprietà

• Origine biologica: synthetic
• Livello qualitativo: 200
• Grado: reagent grade
• Saggio: ≥99% (titration)
• Forma fisica: crystalline
• Condizioni di stoccaggio: (Tightly closed. Dry)
• Impurezze: <0.5% water
• Colore: white
• Intervallo di pH utile: 7.4-8.8
• pKa (25 °C): 8.1
• Punto di fusione: 187 °C
• Solubilità: water: 0.1 M, clear, colorless
• Cationi in tracce: Pb: ≤5 ppm
• Temperatura di conservazione: room temp
• Stringa SMILE: OCC(CO)(CO)NCC(O)=O
• InChI: 1S/C6H13NO5/c8-2-6(3-9,4-10)7-1-5(11)12/h7-10H,1-4H2,(H,11,12); SEQKRHFRPICQDD-UHFFFAOYSA-N

Descrizione

Descrizione generale

Tricine, a zwitterionic biological buffer, is commonly employed in research applications such as enzyme assays, electrophoresis, cell biology, and biochemical studies. Its increased negative charge compared to glycine ensures rapid migration during electrophoresis, facilitating efficient separation of low-molecular-weight proteins in gels with lower acrylamide percentages. Moreover, Tricine may also be used in cryopreservation media, aiding in the preservation of tissues and organs.

Tricine′s valuable properties for biological research include its zwitterionic nature, wide buffering range (pH 7.4-8.8), classification as a Good′s buffering agent, non-toxicity to cells, and high water solubility and clarity. These attributes make it suitable for metal-sensitive systems, stable pH maintenance, optimal biological applications, cell biology studies, and accurate measurements. Additionally, Tricine may find utilization in capillary zone electrophoresis, high-performance liquid chromatography, and ion exchange chromatography, demonstrating adaptability across analytical techniques.

Applicazioni

Tricine is intended to use for:

• Electrophoresis To separate low-weight peptides and proteins
• Crystallization of proteins of varying pH
• Resuspension of cell pellets
• Enzyme assays
• Tissue culture

Componente del tampone per la separazione dei peptidi a basso peso molecolare.

Caratteristiche e vantaggi

• Ideal as a Biological buffer for Cell Biology and Biochemical research
• Effective Buffering from pH 7.4 - 8.8 with a pKa of 8.1 (25 °C)

Altre note

For additional information on our range of Biochemicals, please complete this form.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 11 - Combustible Solids
• Classe di pericolosità dell'acqua (WGK): WGK 3
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable