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Merck
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Key Documents

SRP3016

Sigma-Aldrich

BMP-4 human

recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture

Sinonimo/i:

BMP-2B, DVR4

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About This Item

Codice UNSPSC:
12352202
NACRES:
NA.32

Origine biologica

human

Ricombinante

expressed in E. coli

Saggio

≥98% (HPLC)
≥98% (SDS-PAGE)

Forma fisica

lyophilized

Potenza

5-10 ng/mL ED50

PM

23.8 kDa

Confezionamento

pkg of 10 μg

tecniche

cell culture | mammalian: suitable

Impurezze

<0.1 EU/μg endotoxin, tested

N° accesso UniProt

Condizioni di spedizione

wet ice

Temperatura di conservazione

−20°C

Informazioni sul gene

human ... BMP4(652)

Descrizione generale

Bone morphogenetic proteins (BMPs) constitute a subfamily within the TGF-β (transforming growth factor β) superfamily of structurally related signaling proteins. Escherichia coli derived BMP-4 is a fully active homodimeric protein consisting of two 106 amino acid subunits which correspond to amino acids 303-408 of the full length BMP-4 precursor.

Applicazioni

BMP-4 human has been used for in vitro chondrogenesis in goat adipose tissue-derived mesenchymal stem cells.

Azioni biochim/fisiol

Members of TGF-β (transforming growth factor β) superfamily are widely distributed throughout the body and are involved in diverse physiological processes during both pre- and postnatal life. Like BMP-7, BMP-4 (bone morphogenetic protein 4) is involved in the development and maintenance of bone and cartilage. Reduced expression of BMP-4 is associated with a number of bone diseases, including the heritable disorder Fibrodysplasia Ossificans Progressiva. BMP-4 controls the expression of miRNAs in breast cancer cell lines, thereby regulating the proliferation and migration phenotypes. It is involved in carcinogenesis and exhibits its effect through the SMAD-dependent signaling. In mice, homozygous mutations in BMP4 cause early embryonic lethality.

Sequenza

KKNKNCRRHS LYVDFSDVGW NDWIVAPPGY QAFYCHGDCP FPLADHLNST NHAIVQTLVN SVNSSIPKAC CVPTELSAIS MLYLDEYDKV VLKNYQEMVV EGCGCR

Stato fisico

Lyophilized with no additives.

Ricostituzione

Centrifuge the vial prior to opening.  Reconstitute in 5 mM HCl, pH 3.0 to a concentration of 0.1-1.0 mg/ml.  Do not vortex.  This solution can be stored at 2-8° C for up to 1 week.  For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20° C to -80° C.

Codice della classe di stoccaggio

11 - Combustible Solids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable


Certificati d'analisi (COA)

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I clienti hanno visto anche

Applications of small molecule BMP inhibitors in physiology and disease.
Hong CC and Yu PB
Cytokine & Growth Factor Reviews, 20, 409-418 (2009)
Overexpression of the BMP4/SMAD signaling pathway in skull base chordomas is associated with poor prognosis.
Feng Y
International Journal of Clinical and Experimental Pathology, 8, 8268-8275 (2015)
Bone morphogenetic protein 4 regulates microRNA expression in breast cancer cell lines in diverse fashion.
Alarmo EL
Genes Chromosomes Cancer, 55, 227-236 (2016)
Generation of handmade cloned embryos from adipose tissue derived mesenchymal stem cells in goat.
Malik HN
Small Ruminant Research, 121, 340-350 (2014)
Transgenic mice overexpressing BMP4 develop a fibrodysplasia ossificans progressiva (FOP)-like phenotype.
Kan L
The American Journal of Pathology, 165, 1107-1115 (2004)

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