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Documenti fondamentali

H1404

Sigma-Aldrich

Hepatocyte Growth Factor human

>95% (SDS-PAGE), recombinant, expressed in Baculovirus infected High-5 cells, lyophilized powder, suitable for cell culture

Sinonimo/i:

HGF, Hepatopoietin A, Scatter Factor

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About This Item

Numero CE:
214-275-1
Numero MDL:
MFCD00217422
Codice UNSPSC:
12352202
NACRES:
NA.77

Nome del prodotto

Hepatocyte Growth Factor human, HGF, recombinant, expressed in Baculovirus infected High-5 cells, suitable for cell culture

Origine biologica

human

Livello qualitativo

200

Ricombinante

expressed in Baculovirus infected High-5 cells

Saggio

>95% (SDS-PAGE)

Stato

lyophilized powder

Potenza

1.0-20.0 ng/mL

Qualità

endotoxin tested

PM

~80 kDa

Confezionamento

pkg of 5x5 μg
pkg of 5 μg

Condizioni di stoccaggio

avoid repeated freeze/thaw cycles

tecniche

cell culture | mammalian: suitable

Impurezze

<1.0 EU/μg

N° accesso UniProt

P14210

Temperatura di conservazione

−20°C

Informazioni sul gene

human ... HGF(3082)

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Categorie correlate

Azioni biochim/fisiol

Hepatocyte growth factor (HGF) is a potent mitogen for hepatocytes and a variety of cell types including endothelial and epithelial cells, melanocytes, and keratinocytes. It also mediates epithelial morphogenesis. HGF is the ligand for the tyrosine kinase receptor encoded by the met proto-oncogene.

Altre note

The NSO-expressed recombinant HGF is greater than 95% heterodimeric HGF. The insect cell line-expressed recombinant HGF is a mixture of single chain HGF (predominant) with some heterodimeric HGF. Single chain HGF and heterodimeric HGF are equally active in in vitro assays.

Stato fisico

Lyophilized from a 0.2 μm filtered solution in 20 mM NaH2PO4 and 500 mM NaCl, pH 7.0 with 50 μg BSA per 1 μg HGF

Nota sulla preparazione

Reconstitute at 50 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin. After reconstitution, this solution can be stored at 2-8 °C for up to one month or at -20 °C to -70 °C for up to three months. Us a manual defrost freezer and avoid repeated freeze-thaw cycles.

Risultati analitici

The biological activity is measured by its ability to induce IL-11 secretion by Saos-2 human osieosarcoma cells.

Codice della classe di stoccaggio

11 - Combustible Solids

Classe di pericolosità dell'acqua (WGK)

WGK 2

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Dispositivi di protezione individuale

dust mask type N95 (US), Eyeshields, Gloves

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Certificati d'analisi (COA)

Lot/Batch Number
SLCG7933
SLCF4636
SLCD4440
SLCC0104
SLBZ9529

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Articoli

Hepatocyte Growth Factor (HGF)

Hepatocyte growth factor (HGF) is a potent mitogen for hepatocytes and a variety of other cells, including endothelial and epithelial cells, melanocytes, and keratinocytes.

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