SRE0006
Thymidine Phosphorylase, recombinant from Escherichia coli
recombinant, expressed in E. coli, Suitable for manufacturing of diagnostic kits and reagents, buffered aqueous solution, ≥500 units/mL
Sinonimo/i:
Gliostatins, PD-ECGF, Thymidine:orthophosphate deoxy-D-ribosyltransferase
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About This Item
Numero CAS:
Numero MDL:
Codice UNSPSC:
12352204
NACRES:
NA.54
Prodotti consigliati
Ricombinante
expressed in E. coli
Livello qualitativo
Forma fisica
buffered aqueous solution
Concentrazione
≥500 units/mL
tecniche
inhibition assay: suitable
Colore
colorless to yellow
Solubilità
soluble
water: soluble
N° accesso NCBI
N° accesso UniProt
applicazioni
diagnostic assay manufacturing
Condizioni di spedizione
wet ice
Temperatura di conservazione
2-8°C
Informazioni sul gene
Escherichia coli ... deoA(948901)
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Descrizione generale
Research area: CELL SIGNALING
The E. coli thymidine phosphorylase shares 40% sequence homology with the human sequence, which is identical to the angiogenic agent platelet-derived endothelial growth factor. The purified E. coli enzyme has been shown to stimulate blood vessel growth in chick chorioallantoic membrane assays.
The E. coli thymidine phosphorylase shares 40% sequence homology with the human sequence, which is identical to the angiogenic agent platelet-derived endothelial growth factor. The purified E. coli enzyme has been shown to stimulate blood vessel growth in chick chorioallantoic membrane assays.
Applicazioni
Thymidine phosphorylase has been used:
- in a study to evaluate biomarkers for advanced breast cancer patients treated with capecitabine-based first-line chemotherapy.
- in a study to investigate implications for the clinical efficacy of nucleoside analogues.
Azioni biochim/fisiol
An enzyme that catalyzes the reversible conversion of thymidine to thymine. Thymidine phosphorylase is part of the pyrimidine nucleoside salvage pathway. This pathway allows pyrimidine bases to be recycled for nucleotide biosynthesis, while the pentose 1-phosphates are converted to intermediates of the pentose phosphate shunt and glycolysis. The E. coli thymidine phosphorylase shares 40% sequence homology with the human sequence, which has been found to be identical to the angiogenic agent platelet-derived endothelial growth factor. The purified E. coli enzyme has been shown to stimulate blood vessel growth in chick chorioallantoic membrane assays.
Thymidine phosphorylase catalyzes the reversible conversion of thymidine to thymine. Thymidine phosphorylase is part of the thymidine salvage pathway and pyrimidine nucleoside salvage pathway. This pathway allows pyrimidine bases to be recycled for nucleotide biosynthesis, while the pentose 1-phosphates are converted to intermediates of the pentose phosphate shunt and glycolysis. The enzyme inhibits apoptosis and induces angiogenesis thereby promoting tumor growth and metastatic process. Moreover, thymidine phosphorylase inhibits vascular smooth muscle cell proliferation.
Definizione di unità
One unit will convert 1.0 μmole each of thymidine and phosphate to thymine and 2-deoxyribose 1-phosphate per min at pH 7.4 at 25°C.
Nota sulla preparazione
Cloned from E. coli and produced in overexpressing E. coli
Codice della classe di stoccaggio
12 - Non Combustible Liquids
Classe di pericolosità dell'acqua (WGK)
WGK 2
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
Certificati d'analisi (COA)
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Clinical and biochemical improvements in a patient with MNGIE following enzyme replacement.
Bridget E Bax et al.
Neurology, 81(14), 1269-1271 (2013-08-24)
Akihiko Hatano et al.
Organic & biomolecular chemistry, 11(40), 6900-6905 (2013-09-24)
Herein, we describe β-selective coupling between a modified uracil and a deoxyribose to produce functionalized nucleosides catalyzed by thymidine phosphorylase derived from Escherichia coli. This enzyme mediates nucleobase-exchange reactions to convert unnatural nucleosides possessing a large functional group such as
Thymidine phosphorylase regulates the expression of CXCL10 in rheumatoid arthritis fibroblast-like synoviocytes.
Toyoda Y, Tabata S, Kishi J, et al.
Arthritis & Rheumatology (Hoboken, N.J.), 66(3), 560-568 (2014)
Sheng-hua Zhang et al.
Cancer chemotherapy and pharmacology, 72(4), 777-788 (2013-08-27)
Capecitabine (CAP), a prodrug, needs to be converted to 5-fluorouracil by several key enzymes, including thymidine phosphorylase (TP). To improve the therapeutic index, potentiation of antitumor activity of CAP is required. In this study, we explored whether lidamycin (LDM), an
Hriday Bera et al.
Chemical biology & drug design, 82(3), 351-360 (2013-06-14)
In our lead finding program, a series of 1,2,4-triazolo[1,5-a][1,3,5]triazine derivatives were synthesized, and their in vitro thymidine phosphorylase inhibitory potential was explored. Among the different derivatives, compounds having keto group (C = O) at C7 and thioketo group (C = S) at C5 positions
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