M7257

α-Mannosidase from Canavalia ensiformis (Jack bean)

ammonium sulfate suspension, ≥15 units/mg protein (biuret)

• Sinonimo/i: α-D-Mannoside mannohydrolase
• Numero CAS: 9025-42-7
• Classificazione EC (Enzyme Commission): 3.2.1.24 (BRENDA, IUBMB)
• Numero CE: 232-794-1
• Numero MDL: MFCD00130495
• Codice UNSPSC: 12352204
• NACRES: NA.54

Proprietà

• Origine biologica: Canavalia ensiformis
• Livello qualitativo: 200
• Forma fisica: ammonium sulfate suspension
• Attività specifica: ≥15 units/mg protein (biuret)
• Attività estranea: β-galactosidase, β-N-acetylglucosaminidase, α-galactosidase and α-L-fucosidase <0.05%
• Temperatura di conservazione: 2-8°C
• InChI: 1S/Zn/q+2; PTFCDOFLOPIGGS-UHFFFAOYSA-N

Descrizione

Descrizione generale

α−Mannosidase from Canavalia ensiformis is a tretamer composed of two subunits that each contain two components at 44 and 66 kDa.

α-Mannosidase is an exo-glycosidase and an inc metalloenzyme.

Lysosomal α-mannosidases are class II mannosidases that belong to glycoside hydrolase family 38 and play an important role in the degradation of asparagine-linked carbohydrates of glycoproteins.

Applicazioni

α-Mannosidase can be used to liberate mannose from a variety of synthetic and natural α-mannosides. It has also been used in a study to investigate the causes of neurodegeneration in mucolipidosis II ′knock-in′ mice.

α-Mannosidase from Canavalia ensiformis (Jack bean) has been used:

• for the digestion of neutral glycans.
• to remove O-glycans from recombinant β-glucosidase
• for the inhibition of renal fibroblasts proliferation

Liberates mannose from a variety of synthetic and natural α-mannosides.

Azioni biochim/fisiol

α−Mannosidase has been shown to inhibit the proliferation of B-lymphocytes.

α-Mannosidase cleaves near α1−2-, α1−3- and α1−6-linked mannose residues.

α-Mannosidase is an acid hydrolase which is located in plant vacuoles and is thought to be involved with the turnover of N-linked glycoproteins.

Definizione di unità

One unit will hydrolyze 1.0 μmole of p-nitrophenyl α-D-mannoside to p-nitrophenol and D-mannose per min at pH 4.5 at 25°C.

Stato fisico

Suspension in 3.0 M (NH₄)₂SO₄ and 0.1 mM zinc acetate, pH 7.5

Informazioni sulla sicurezza

• Pittogrammi: GHS08
• Avvertenze: Danger
• Indicazioni di pericolo: H334
• Consigli di prudenza: P261 - P284 - P501
• Classi di pericolo: Resp. Sens. 1
• Codice della classe di stoccaggio: 11 - Combustible Solids
• Classe di pericolosità dell'acqua (WGK): WGK 1
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable
• Dispositivi di protezione individuale: Eyeshields, Gloves, type N95 (US)