L4025
Liticasi
lyophilized powder, ≥200 units/mg solid
Sinonimo/i:
(1,3)-β-D-Glucan endohydrolase, 1,3-β-Glucan glucohydrolase, Bacterial lyticase, Lysing enzyme
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About This Item
Origine biologica
bacterial (Arthrobacter luteus)
Livello qualitativo
Forma fisica
lyophilized powder
Attività specifica
≥200 units/mg solid
tecniche
cell based assay: suitable
Compatibilità
suitable for cell lysis
applicazioni
diagnostic assay manufacturing
Temperatura di conservazione
−20°C
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Applicazioni
Lyticase from Arthrobacter luteus has been used for resuspending cells to extract high-molecular weight DNA for polymerase chain reaction (PCR) fingerprint analyses.
Lyticase from Arthrobacter luteus has been used:
- as a component in spheroplasting buffer to prepare yeast nuclear extracts
- for digestion in sample preparation for β-glucan enzymatic assay using yeast
- to lyse cells for RNA isolation
Azioni biochim/fisiol
Lyticase enzyme is frequently used in fungal research, particularly for species identification using polymerase chain reaction (PCR)-based techniques. It can break down β (1→3) and β (1→4) bonds between glucose units.
Lyticase is a lysing enzyme that is used to extract DNA from yeast cells by inducing partial spheroplast formation. Spheroplasts are subsequently lysed to release DNA. Lyticase is preferred to digest cell walls of yeast, which are difficult to disrupt because the cell walls may form capsules or resistant spores. Lyticase contains β-(1→;3)-glucan laminaripentaohydrolase along with β-(1→3)-glucanase, protease, and mannanase activities. It is useful with yeast cells such as e.g. Candida, Debaryomyces, Saccharomyces, Saccharomycopsis, Saccharomycodes, Eremothecium, and Schwanniomyces species.
La liticasi idrolizza il poli-β(1→3)-glucosio come ad esempio il glucano delle pareti cellulari del lievito.
Definizione di unità
Un′unità produce una ΔA800 di 0,001 al minuto a pH 7,5 a 25 °C usando una sospensione di lievito come substrato in 3 mL di miscela di reazione.
Altre note
Per ulteriori informazioni sugli enzimi per l′analisi dei carboidrati complessi, visitare il sito web www.sigma-aldrich.com/enzymeexplorer.
For R&D use only. Not for drug, household, or other uses. Please consult the Safety Data Sheet for information regarding hazards and safe handling practices.
Avvertenze
Danger
Indicazioni di pericolo
Consigli di prudenza
Classi di pericolo
Resp. Sens. 1
Codice della classe di stoccaggio
11 - Combustible Solids
Classe di pericolosità dell'acqua (WGK)
WGK 3
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
Dispositivi di protezione individuale
Eyeshields, Gloves, type N95 (US)
Certificati d'analisi (COA)
Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.
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Protocolli
This procedure may be used for the determination of Lyticase activity using Baker’s yeast as the substrate.
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