SAE0049

Lactic Dehydrogenase, recombinant

from human, recombinant, expressed in E. coli, aqueous solution

• Sinonimo/i: (S)-Lactate: NAD⁺ oxidoreductase, L-Lactate Dehydrogenase
• Numero CAS: 9001-60-9
• Classificazione EC (Enzyme Commission): 1.1.1.27 (BRENDA, IUBMB)
• Numero MDL: MFCD00131466
• Codice UNSPSC: 12352200
• NACRES: NA.54

Proprietà

• Origine biologica: human
• Livello qualitativo: 200
• Ricombinante: expressed in E. coli
• Forma fisica: aqueous solution
• Condizioni di stoccaggio: (Keep container tightly closed in a dry and well-ventilated place)
• Colore: colorless
• N° accesso UniProt: K1T0A2
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: −20°C
• Informazioni sul gene: human ... LDHA(3939)

Descrizione

Descrizione generale

Research area: Cell Signaling
The gene LDHA (L-lactate dehydrogenase A chain) is mapped to human chromosome 11p15. It is a subunit of lactate dehydrogenase.In particular, lactic dehydrogenase A (LDHA) is mainly found in skeletal muscle, and for that reason is known as the M subunit. This recombinant form of LDHA has a C-terminal histidine-tag.

Applicazioni

L-Lactate Dehydrogenase (LDHA) has been used in in vitro phosphoglycerate mutase 1 (PGAM1) inhibitors screening assay. It has also been used in a colorimetric assay for determining lactate concentration in conditioned media.

Azioni biochim/fisiol

L-lactic dehydrogenase catalyzes the conversion of L-lactate into L-pyruvate while reducing NAD⁺ to NADH and H⁺.  L-lactate dehydrogenase A chain (LDHA), an enzyme involved in pyruvate metabolism, LDH is responsible for the conversion of pyruvate to lactate, the final step of glycolysis. It is overexpressed in various cancers. LDHA regulates the microenvironment of developing tumors by the hypoxia-inducible factor (HIF)-signaling pathway. LDHA aids in the NAD⁺ regeneration during the β-oxidation of fatty acid. LDHA (L-lactate dehydrogenase A chain) is responsible for the conversion of pyruvate to lactate, the final step of glycolysis. It is overexpressed in various cancers. In cancer cells, HIF-1a (hypoxia-inducible factor) induces the expression of LDHA, which helps in maintaining glycolysis in cells.

L-lactic dehydrogenase catalyzes the conversion of L-lactate into L-pyruvate while reducing NAD⁺ to NADH and H⁺.

Definizione di unità

One unit will reduce 1.0 μmole of pyruvate to L-lactate per min at pH 7.5 at 37 °C.

Stato fisico

Buffered aqueous solution with Hepes (pH 7.5), NaCl and glycerol.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 10 - Combustible liquids
• Classe di pericolosità dell'acqua (WGK): WGK 1
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable