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Merck
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Key Documents

SAB4301135

Sigma-Aldrich

Anti-FLAG Tag antibody produced in rabbit

affinity isolated antibody

Sinonimo/i:

Anti-ddddk, Anti-dykddddk

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About This Item

Codice UNSPSC:
12352203
NACRES:
NA.43

Origine biologica

rabbit

Livello qualitativo

Coniugato

unconjugated

Forma dell’anticorpo

affinity isolated antibody

Tipo di anticorpo

primary antibodies

Clone

polyclonal

Forma fisica

buffered aqueous solution

Reattività contro le specie

all

Concentrazione

1.4 mg/mL

tecniche

western blot: 1:1000-1:5000 (Cell Lysate)

Isotipo

IgG

Sequenza immunogenica

DYKDDDDK conjugated to KLH (synthetic peptide)

Condizioni di spedizione

wet ice

Temperatura di conservazione

−20°C

Descrizione generale

The FLAG epitope is composed of eight amino acids with the following sequence AspTyrLysAspAspAspAspLys. It helps in immunoaffinity purification of fusion proteins.

Specificità

The antibody detects transfected proteins contanining FLAG tag.

Immunogeno

Synthetic peptide: DYKDDDDK conjugated to KLH

Applicazioni

Anti-FLAG Tag antibody produced in rabbit has been used in:
  • western blotting
  • co-immunoprecipitation
  • immunofluorescence studies
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)
Learn more product details in our FLAG® application portal.

Caratteristiche e vantaggi

Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.

Stato fisico

Rabbit IgG in pH7.3 PBS, 0.05% NaN3, 50% Glycerol.

Note legali

FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Codice della classe di stoccaggio

10 - Combustible liquids

Classe di pericolosità dell'acqua (WGK)

WGK 1

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable


Certificati d'analisi (COA)

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Amelia C McKitterick et al.
Cell host & microbe, 26(4), 504-514 (2019-10-11)
Phage defense systems are often found on mobile genetic elements (MGEs), where they constitutively defend against invaders or are induced to respond to new assaults. Phage satellites, one type of MGE, are induced during phage infection to promote their own
Yunlan Tang et al.
Biochemical and biophysical research communications, 518(2), 396-401 (2019-08-21)
Nuclear Dbf2-related kinase 2 (NDR2) is a highly conserved kinase that belongs to the NDR/LATS serine-threonine kinase family. NDR2 is involved in many cellular processes as a kinase or a scaffolding protein. As a known kinase, NDR2 requires self-phosphorylation and
Xuan Yang et al.
Cancer science, 110(7), 2133-2144 (2019-05-09)
Lymphoid-specific helicase (LSH) is overexpressed in tumor tissues and its overexpression is associated with poor prognosis in several cancers. However, the role and molecular mechanism of LSH in hepatocellular carcinoma (HCC) remains largely unknown. Herein, we report that LSH was
Yersinia YopJ negatively regulates IRF3-mediated antibacterial response through disruption of STING-mediated cytosolic DNA signaling
Cao Y, et al.
Biochimica et Biophysica Acta, 1863(12), 3148-3159 (2016)
Yun Teng et al.
Molecular therapy : the journal of the American Society of Gene Therapy, 29(8), 2424-2440 (2021-05-14)
Lung inflammation is a hallmark of coronavirus disease 2019 (COVID-19). In this study, we show that mice develop inflamed lung tissue after being administered exosomes released from the lung epithelial cells exposed to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)

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