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SAB4200809

Sigma-Aldrich

Anti-Epidermal Growth Factor (EGF) Receptor, Mouse monoclonal

clone 29.1, purified from hybridoma cell culture

Sinonimo/i:

EGFR, Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1

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About This Item

Codice UNSPSC:
12352203
NACRES:
NA.41

Origine biologica

mouse

Forma dell’anticorpo

purified from hybridoma cell culture

Tipo di anticorpo

primary antibodies

Clone

29.1, monoclonal

Forma fisica

buffered aqueous solution

PM

~170 kDa

Reattività contro le specie

human, bovine, mouse

Confezionamento

antibody small pack of 25 μL

Concentrazione

~1 mg/mL

tecniche

immunoblotting: 0.5-1 μg/mL using human epidermoid carcinoma A431 cell line extract.
immunofluorescence: 2-4 μg/mL using human epidermoid carcinoma A431 cells.

Isotipo

IgG1

N° accesso UniProt

P00533

Condizioni di spedizione

dry ice

Temperatura di conservazione

−20°C

modifica post-traduzionali bersaglio

unmodified

Informazioni sul gene

human ... EGFR(1956)

Descrizione generale

Epidermal growth factor receptor (EGFR), also known as Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1 or HER-1, is an integral cell membrane protein which spans the membranes of a wide range of normal and malignant epithelial cells. EGFR is a tyrosine-specific protein kinase domain, it has an extracellular region which binds EGF and an intra-cellular carboxy-terminal region which possesses the tyrosine kinase activity. EGF binding to its specific EGFR, results in an increased DNA synthesis initiating a signaling cascades including cell proliferation, differentiation and repair of damaged epithelial tissue.

Immunogeno

Paraformaldehyde-fixed carcinoma cell line A431

Applicazioni

Monoclonal Anti-Epidermal Growth Factor (EGF) Receptor specifically bind to a carbohydrate residue on the external region of the EGF receptor molecule with no interference to the binding of EGF to its receptor, nor does it influence the binding of other monoclonal antibodies to the EGF Receptor. Reactivity has been observed with EGF receptors from mouse, human and bovine origin. The antibody is recommended to use in various immunological techniques, including Immunobloting (~170 kDa), Immunoprecipitation, flow cytometry (FACS), Immunohistochemistry and Immunofluorescence.

Stato fisico

Supplied as a solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide as a preservative.

Altre note

This product is for R&D use only, not for drug, household, or other uses.

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Codice della classe di stoccaggio

10 - Combustible liquids

Classe di pericolosità dell'acqua (WGK)

WGK 1

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

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E Gerdin et al.
American journal of clinical pathology, 99(1), 28-31 (1993-01-01)
It has been reported that endothelium in malignant glioma stains with a commercial antibody raised against the receptor for epidermal growth factor (EGFr) on A431 cells (clone 29.1). In this report, this antibody was used to study the immunohistochemical expression
Y A Kita et al.
FEBS letters, 349(1), 139-143 (1994-07-25)
Her3/erbB3 has been identified as a third member of the epidermal growth factor receptor (EGFR) family [(1989) Proc. Natl. Acad. Sci. USA 86, 9193-9197; (1990) Proc. Natl. Acad. Sci. USA 87, 4905-4909]. The natural ligand for Her3 has not been
L H Defize et al.
The Journal of cell biology, 107(3), 939-949 (1988-09-01)
The epidermal growth factor receptor (EGF-R) of human A431 cells bears an antigenic determinant that is closely related to the human blood group A carbohydrate structure. Labeling studies with blood group A reactive anti-EGF-R monoclonal antibodies and various lectins revealed
Y Yarden et al.
The Journal of biological chemistry, 260(1), 315-319 (1985-01-10)
A method is described for a rapid two-step purification of the membrane receptor for epidermal growth factor (EGF) from cultured human A-431 cells. After solubilization of the cells with Triton X-100, the receptor is immobilized on an immunoaffinity column containing
Alexander Sorkin et al.
Current protocols in cell biology, Chapter 15, Unit 15-Unit 15 (2010-03-18)
Binding of epidermal growth factor (EGF) to the EGF receptor (EGFR) initiates signal transduction, ultimately leading to altered gene expression. Ligand-activated EGFR is also rapidly internalized and then targeted to lysosomes for degradation or recycled back to the plasma membrane.
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