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Key Documents

SAB4200100

Sigma-Aldrich

Anti-ATG13 antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody

Sinonimo/i:

ATG13 Antibody - Anti-ATG13 antibody produced in rabbit, Atg13 Antibody, Anti-Autophagy-related protein 13

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About This Item

Codice UNSPSC:
12352203
NACRES:
NA.41

Origine biologica

rabbit

Coniugato

unconjugated

Forma dell’anticorpo

affinity isolated antibody

Tipo di anticorpo

primary antibodies

Clone

polyclonal

Forma fisica

buffered aqueous solution

PM

antigen ~65 kDa

Reattività contro le specie

human, rat, mouse

Concentrazione

~1.0 mg/mL

tecniche

western blot: 1-2 μg/mL using whole extracts of human HeLa cells and 0.5-1 μg/mL using whole extracts of mouse 3T3 or rat PC12 cells.

Condizioni di spedizione

dry ice

Temperatura di conservazione

−20°C

modifica post-traduzionali bersaglio

unmodified

Informazioni sul gene

human ... KIAA0652(9776)

Categorie correlate

Descrizione generale

Autophagy-related protein 13 (Atg13) is mapped to human chromosome 11p11.2. It comprises of HORMA domain (Hop1, Rev7 and MAD2).

Specificità

Anti-Atg13 recognizes human, mouse, and rat Atg13.

Applicazioni

Anti-ATG13 antibody produced in rabbit has been used in immunoblotting and immunofluorescence microscopy.

Azioni biochim/fisiol

Autophagy-related protein 13 (Atg13) is essential for autophagosome formation in mammalian cells. Atg13 forms a stable complex with ULK1 (Unc-51 Like Autophagy Activating Kinase 1) and FAK family-interacting protein of 200 kDa (FIP200). Mammalian target of rapamycin (mTOR) interacts with this complex in a nutrient dependent manner and phosphorylates Atg13 and ULK1, suggesting that mTOR regulates autophagy through the ULK1-Atg13-FIP200 complex.

Stato fisico

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Stoccaggio e stabilità

Store at –20 °C. For continuous use, store at 2–8 °C for up to one month. For extended storage, freeze in working aliquots at –20 °C. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Codice della classe di stoccaggio

12 - Non Combustible Liquids

Classe di pericolosità dell'acqua (WGK)

nwg

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable


Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

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Autophagosome formation is initiated at phosphatidylinositol synthase-enriched ER subdomains
Nishimura T, et al.
The Embo Journal, 36(12), 1719-1735 (2017)
Di Ge et al.
Autophagy, 10(6), 957-971 (2014-06-01)
MTOR, a central regulator of autophagy, is involved in cancer and cardiovascular and neurological diseases. Modulating the MTOR signaling balance could be of great significance for numerous diseases. No chemical activators of MTOR have been found, and the urgent challenge
Selective Autophagy of Mitochondria on a Ubiquitin-Endoplasmic-Reticulum Platform
Zachari M, et al.
Developmental Cell (2019)
Systematic analysis of ATG13 domain requirements for autophagy induction
Wallot-Hieke N, et al.
Autophagy, 14(5), 743?763-743?763 (2018)
Physical and neurobehavioral determinants of reproductive onset and success
Day FR, et al.
Nature Genetics, 48(6), 617-617 (2016)

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