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RAB0436

Sigma-Aldrich

Human Osteopontin ELISA Kit

for serum, plasma, cell culture supernatant and urine

Sinonimo/i:

OPN

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About This Item

Codice UNSPSC:
41116158
NACRES:
NA.32

Reattività contro le specie

human

Confezionamento

kit of 96 wells (12 strips x 8 wells)

tecniche

ELISA: suitable
capture ELISA: suitable

input

sample type serum
sample type plasma
sample type urine
sample type cell culture supernatant(s)

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 50 pg/mL
standard curve range: 74.07-18000 pg/mL

Metodo di rivelazione

colorimetric

Condizioni di spedizione

wet ice

Temperatura di conservazione

−20°C

Informazioni sul gene

human ... SPP1(6696)

Categorie correlate

Descrizione generale

The Human OPN ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human OPN in serum, plasma, cell culture supernatants and urine.

Immunogeno

Recombinant Human OPN

Applicazioni

For research use only. Not for use in diagnostic procedures.
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)
Human Osteopontin ELISA Kit has been used in the quantification of serum osteopontin (OPN) in human blood samples.

Azioni biochim/fisiol

Osteopontin (OPN) is a secreted glycoprotein and is assumed to be associated with various cellular processes such as adhesion, migration, and biomineralization in diverse tissues. Osteopontin acts as a ligand to αvβ3 integrin and possibly other receptors. In addition to its involvement in mineralized matrix formation, osteopontin can also function as a cytokine that stimulates the release of IFNγ (interferon γ) and IL-12 (interleukin 12), while inhibiting the production of IL-10. Osteopontin is involved in inflammation and also regulates immunity against infectious diseases. It also co-stimulates T cell proliferation. Osteopontin has proinflammatory functions and is associated with multiple sclerosis. It participates in inflammation in Crohn′s Disease. Osteopontin plays a crucial role in bone remodelling and retards ectopic calcification by inhibiting the formation of hydroxyapatite. OPN is thought to prevent renal stone formation by suppressing nucleation, growth and aggregation of calcium oxalate crystals.

Altre note

A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.

I componenti del kit sono disponibili anche separatamente

N° Catalogo
Descrizione
SDS
  • RABELADEELISA 5X Assay/Sample Diluent Buffer E (Item E2)SDS
  • RABSTOP3ELISA Stop Solution (Item I)SDS
  • RABTMB3ELISA Colorimetric TMB Reagent (HRP Substrate, Item H)SDS
  • RABWASH420X Wash Buffer (Item B)SDS

Pittogrammi

Corrosion
GHS05

Avvertenze

Warning

Indicazioni di pericolo

H290

Consigli di prudenza

P234 - P390

Classi di pericolo

Met. Corr. 1

Codice della classe di stoccaggio

8A - Combustible corrosive hazardous materials

Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

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Can Osteopontin Be Considered a Biomarker for Endometriosis?
Albu D R, et al.
Revista de Chimie (Bucharest, Romania), 13(3), 1-1 (2017)
Octavio Gamaliel Aztatzi-Aguilar et al.
PloS one, 16(3), e0249057-e0249057 (2021-03-26)
Osteopontin (OPN) is a protein involved in inflammatory illnesses such as fibrosis and cancer; its overexpression in cardiovascular diseases promotes the biomineralization of blood vessels and other soft tissues. Moreover, there is an active component of oxidative stress related with
Post-translational modification and proteolytic processing of urinary osteopontin.
Christensen B, et al.
The Biochemical Journal, 411, 53-61 (2008)
The adhesive and migratory effects of osteopontin are mediated via distinct cell surface integrins. Role of alpha v beta 3 in smooth muscle cell migration to osteopontin in vitro.
Liaw L, et al.
The Journal of Clinical Investigation, 95, 713-724 (1995)
The influence of the proinflammatory cytokine, osteopontin, on autoimmune demyelinating disease.
Chabas D, et al.
Science, 294, 1731-1735 (2001)
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