C0775

Crystal Violet

Dye content, ≥90%, certified by the Biological Stain Commission, powder

• Sinonimo/i: Basic Violet 3, Gentian Violet, Hexamethylpararosaniline chloride, Methyl Violet 10B
• Formula condensata: C₂₅H₃₀N₃Cl
• Numero CAS: 548-62-9
• Peso molecolare: 407.98
• Colour Index: 42555
• Beilstein: 3580948
• Numero CE: 208-953-6
• Numero MDL: MFCD00011750
• Codice UNSPSC: 12171500
• ID PubChem: 57653980
• NACRES: NA.47

Proprietà

• Nome del prodotto: Crystal Violet, certified by the Biological Stain Commission
• Grado: certified by the Biological Stain Commission
• Stato: powder
• Composizione: Dye content, ≥90%
• tecniche: microbe id | staining: suitable
• Colore: green to very dark green
• pH: 2.5 — 3.5 (20 °C)
• Punto di fusione: 205 °C (dec.) (lit.)
• Solubilità: H₂O: 1 mg/mL
• Densità: 1.19 g/cm³ at 20 °C
• Spettro attività antibiotica: fungi
• applicazioni: diagnostic assay manufacturing; hematology; histology
• Modalità d’azione: cell membrane | interferes; enzyme | inhibits
• Temperatura di conservazione: room temp
• Stringa SMILE: [Cl-].CN(C)c1ccc(cc1)\C(c2ccc(cc2)N(C)C)=C3/C=C\C(C=C3)=[N+](/C)C
• InChI: 1S/C25H30N3.ClH/c1-26(2)22-13-7-19(8-14-22)25(20-9-15-23(16-10-20)27(3)4)21-11-17-24(18-12-21)28(5)6;/h7-18H,1-6H3;1H/q+1;/p-1; ZXJXZNDDNMQXFV-UHFFFAOYSA-M

Descrizione

Applicazioni

Crystal violet is used as an active component, primary stain, of Gram stain for differentiation of Gram-negative versus Gram-positive bacteria.
It has been used to check cell viability. It has also been used for the staining of cells to study cell migration and invasion.

Azioni biochim/fisiol

Crystal violet can be used for DNA visualization in agarose gels. The dye is used only in the presence of high concentrations of DNA. Crystal violet is also used for the staining of bacteria in gram staining technique. It is also used for the staining of plant chromosomes. Crystal Violet also helps in colorimetric measurement of cell viability.

Compatibilità

Certified for use with the Gram stain for bacteria, Flemming triple stain with iodine for chromosomes, Lieb′s amyloid stain and Newton′s crystal violet-iodine technique for chromatin and nucleoli.

Informazioni sulla sicurezza

• Pittogrammi: GHS08,GHS05,GHS07,GHS09
• Avvertenze: Danger
• Indicazioni di pericolo: H302,H318,H351,H410
• Consigli di prudenza: P202 - P273 - P280 - P301 + P312 - P305 + P351 + P338 - P308 + P313
• Classi di pericolo: Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Carc. 2 - Eye Dam. 1
• Codice della classe di stoccaggio: 11 - Combustible Solids
• Classe di pericolosità dell'acqua (WGK): WGK 3
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable