RAB0408

Mouse Prolactin ELISA Kit

for serum, plasma and cell culture supernatant

• Codice UNSPSC: 41116158
• NACRES: NA.84

Proprietà

• Reattività contro le specie: mouse
• Confezionamento: kit of 96 wells (12 strips x 8 wells)
• tecniche: ELISA: suitable; capture ELISA: suitable
• input: sample type plasma; sample type serum; sample type cell culture supernatant(s)
• assay range: inter-assay cv: <12%; intra-assay cv: <10%; sensitivity: 30 pg/mL; standard curve range: 27.43-20000 pg/mL
• Metodo di rivelazione: colorimetric
• Condizioni di spedizione: wet ice
• Temperatura di conservazione: −20°C
• Informazioni sul gene: mouse ... Prl(19109)

Descrizione

Descrizione generale

The Mouse Prolactin ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse Prolactin in serum, plasma, cell culture supernatants and urine.

Immunogeno

Recombinant Mouse Prolactin

Applicazioni

For research use only. Not for use in diagnostic procedures.
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)

Azioni biochim/fisiol

Prolactin (Prl) is processed to lower molecular weight N-terminal fragments, 14-18kDa, called vasoinhibins, which have antiangiogenic effects. In endothelial cells, the 16kDa prolactin form exerts antiproliferative and proapoptotic effects which result in cell cycle arrest, caspase and NFκB (nuclear factor) activation and regulation of Bcl-2 protein family. In sexually mature female mice, the plasma levels of prolactin remain constant throughout estrous cycle, but show a sudden increase during proestrous cycle due to increase in circulating levels of estrogens. Studies show that Prl activates T cells, B cells, natural killer (NK) cells, macrophages, neutrophils and dendritic cells and there is a significant level of hyperprolactinemia incidence in patients with autoimmune diseases, such as rheumatoid arthritis (RA) and systemic lupus erythematosus (SLE). Studies in rats show that Prl is involved in T cell homing to mammary glands during early lactation and that hypoprolactemia during lactation results in modified T cell distribution with increased T cell in circulation and reduced in mammary glands.

Altre note

A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.

I componenti del kit sono disponibili anche separatamente

• RABELADA ELISA 1X Assay/Sample Diluent Buffer A (Item D1)
• RABELADB ELISA 5X Assay/Sample Diluent Buffer B (Item E1)
• RABELADC ELISA 1X Assay/Sample Diluent Buffer C (Item L)
• RABSTOP3 ELISA Stop Solution (Item I)
• RABTMB3 ELISA Colorimetric TMB Reagent (HRP Substrate, Item H)
• RABWASH4 20X Wash Buffer (Item B)

Informazioni sulla sicurezza

• Pittogrammi: GHS05
• Avvertenze: Warning
• Indicazioni di pericolo: H290
• Consigli di prudenza: P234 - P390
• Classi di pericolo: Met. Corr. 1
• Codice della classe di stoccaggio: 8A - Combustible corrosive hazardous materials