R6267

RANTES human

≥97% (SDS-PAGE), recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture

• Sinonimo/i: Regulated on Activation, Normal T-cell Expressed and Secreted
• Numero CE: 200-835-2
• Numero MDL: MFCD00213042
• Codice UNSPSC: 12352202
• NACRES: NA.32

Proprietà

• Origine biologica: human
• Livello qualitativo: 100
• Ricombinante: expressed in E. coli
• Saggio: ≥97% (SDS-PAGE)
• Stato: lyophilized powder
• Potenza: 1-5 ng/mL EC₅₀
• PM: 7.8-8.7 kDa
• Confezionamento: pkg of 10 μg
• Condizioni di stoccaggio: avoid repeated freeze/thaw cycles
• tecniche: cell culture | mammalian: suitable
• Impurezze: endotoxin, tested
• N° accesso UniProt: P13501
• Temperatura di conservazione: −20°C
• Informazioni sul gene: human ... CCL5(6352)

Descrizione

Descrizione generale

Regulated on activation, normal T cell expressed and secreted (RANTES), also known as C-C motif chemokine ligand 5 (CCL5), is encoded by the gene mapped to human chromosome 17. RANTES belongs to the chemokine family of proinflammatory basic polypeptides and is expressed by various hematopoietic and non-hematopoietic cell types involved in homing and migration of effector and memory T cells during acute infections.

Azioni biochim/fisiol

Regulated on activation, normal T cell expressed and secreted (RANTES) is a chemoattractant towards monocytes, dendritic cells (DCs), memory T cells (CD4⁺/CD45RO), basophils, and eosinophils. The encoded chemokine protein functions as a ligand for CCR1, CCR3, and CCR5. RANTES inhibits the action of R5 strains of HIV-1 via blocking CCR5 receptor. A variant of RANTES gene is linked with susceptibility to HIV-1 infection and disease progression. Hence, this can be used as a potent biomarker to determine the prognosis of HIV -1 infection.

Stato fisico

Lyophilized from a 0.2 μm filtered solution in 35% acetonitrile and 0.1% trifluoroacetic acid containing 0.5 mg bovine serum albumin.

Risultati analitici

The biological activity is tested in culture using Baf/3 cells. Chemotaxis: EC₅₀: 1-5 ng/mL

Informazioni sulla sicurezza

• Pittogrammi: GHS02,GHS07
• Avvertenze: Danger
• Indicazioni di pericolo: H225,H302 + H312 + H332,H319
• Consigli di prudenza: P210 - P280 - P301 + P312 - P303 + P361 + P353 - P304 + P340 + P312 - P305 + P351 + P338
• Classi di pericolo: Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Eye Irrit. 2 - Flam. Liq. 2
• Codice della classe di stoccaggio: 3 - Flammable liquids
• Classe di pericolosità dell'acqua (WGK): WGK 2
• Punto d’infiammabilità (°F): 35.6 °F
• Punto d’infiammabilità (°C): 2 °C