P4524

β-Lactamase from Enterobacter cloacae

Type IV, lyophilized powder, 0.2-0.6 units/mg protein (using benzylpenicillin)

• Sinonimo/i: β-Lactamase I, β-Lactamase II, Cephalosporinase, Penicillin amido-β-lactam hydrolase, Penicillinase from Enterobacter cloacae
• Numero CAS: 9073-60-3
• Classificazione EC (Enzyme Commission): 3.5.2.6 (BRENDA, IUBMB)
• Numero MDL: MFCD00131819
• Codice UNSPSC: 12352204
• NACRES: NA.54

Proprietà

• Origine biologica: bacterial (Enterobacter cloacae)
• Livello qualitativo: 200
• Tipo: Type IV
• Stato: lyophilized powder
• Attività specifica: 0.2-0.6 units/mg protein (using benzylpenicillin)
• PM: 29 kDa
• Composizione: Protein, ~60%
• tecniche: enzyme immunoassay: suitable
• Compatibilità: suitable for enzyme test
• applicazioni: pharmaceutical
• Temperatura di conservazione: 2-8°C

Descrizione

Descrizione generale

β-Lactamase is a monomeric enzyme of 29 kDa. This product is produced from Enterobacter cloacae and is provided as a lyophilized powder. It acts as a target protein for β-lactam antibiotics. This enzyme is present in Gram-negative bacteria.

Applicazioni

β--lactamase is used to inactivate β-lactam antibiotics by breaking open the β-lactam ring. β--lactamase is used to study antibiotic resistance and resistance suppression. Product P4524 is produced from Enterobacter cloacae and is provided as a lyophilized powder.

β-Lactamase from Enterobacter cloacae has been used in the maternal and fetal quantitative blood cultures to avoid any carryover phenomenon. It has also been used as a component in Mueller Hinton (MH) broth for placental cultures.

Azioni biochim/fisiol

β--lactamase inactivates β-lactam antibiotics by breaking open the β-lactam ring.

Definizione di unità

One unit will hydrolyze 1.0 μmole of benzylpenicillin per min at pH 7.0 at 25 °C. This International Unit (using benzylpenicillin as substrate) is approximately equal to 600 Levy or 75 Pollock units.

Stato fisico

Lyophilized powder containing sodium phosphate buffer salts

Risultati analitici

Protein determined by biuret.

Informazioni sulla sicurezza

• Pittogrammi: GHS08
• Avvertenze: Danger
• Indicazioni di pericolo: H317,H334
• Consigli di prudenza: P261 - P272 - P280 - P284 - P302 + P352 - P333 + P313
• Classi di pericolo: Resp. Sens. 1 - Skin Sens. 1
• Codice della classe di stoccaggio: 11 - Combustible Solids
• Classe di pericolosità dell'acqua (WGK): WGK 3
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable
• Dispositivi di protezione individuale: dust mask type N95 (US), Eyeshields, Faceshields, Gloves