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P1261

Sigma-Aldrich

Phosphorylase a from rabbit muscle

lyophilized powder, 20-30 units/mg protein

Sinonimo/i:

1,4-α-D-Glucan:orthophosphate α-D-glucosyltransferase

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About This Item

Numero CAS:
9032-10-4
Classificazione EC (Enzyme Commission):
2.4.1.1 (BRENDA, IUBMB)
Numero CE:
232-878-8
Numero MDL:
MFCD00167428
Codice UNSPSC:
12352204
NACRES:
NA.54

Origine biologica

rabbit muscle

Forma fisica

lyophilized powder

Attività specifica

20-30 units/mg protein

Purificato mediante

2× crystallization

Composizione

Protein, 30-60% E1%/280

Attività estranea

AMP-phosphatase, ATP-phosphatase, debrancher enzyme and phosphorylase phosphatase ≤0.1%
phosphoglucomutase ≤0.25%
phosphorylase b ≤30%

Temperatura di conservazione

−20°C

Descrizione generale

Phosphorylase A is the active form of glycogen phosphorylase which converts glycogen and orthophosphate (Pi) to glucose 1-phoshate (G-1-P).

Applicazioni

Phosphorylase a from rabbit muscle has been used to mix with glycogen for the preparation of limit dextrin. It has also been used to probe the array in oligosaccharide microarray analysis of phosphorylase activity.
Phosphorylase from rabbit muscle has been used in a study to assess the molecular mechanisms of oleanolic acid. It has also been used in a study to describe the conversion of phosphorylase B to A, through a conversion enzyme in the presence of 32P-ATP.

Azioni biochim/fisiol

Dimeric phosphorylase b is converted to the more active tetramer, phosphorylase a, by the action of phosphorylase kinase.
Phosphorylase A can be inhibited by these compounds: Polychlorinated biphenyls, polychlorinated biphenylols and polybrominated biphenyls.
Phosphorylase participates in glycogenolysis. Deficiency of phosphorylase leads to several glycogen storage diseases with hepatomegaly.

Definizione di unità

One unit will form 1.0 μmole of α-D-glucose 1-phosphate from glycogen and orthophosphate per min at pH 6.8 at 30 °C, measured in a system containing phosphoglucomutase, NADP, and glucose-6-phosphate dehydrogenase. (One μmolar unit is equivalent to ~45 Cori units.)

Stato fisico

Lyophilized powder containing β-glycerophosphate and EDTA

Codice della classe di stoccaggio

11 - Combustible Solids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Dispositivi di protezione individuale

Eyeshields, Gloves, type N95 (US)

Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

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Hypoglycemia in the toddler and child
Pediatric Endocrinology, Diabetes, and Metabolism, 920-955 (2014)
T C Chang et al.
Plant physiology, 80(2), 534-538 (1986-02-01)
A protein, starch phosphorylase inhibitor, was purified from the root of sweet potato (Ipomoea batatas [L.] Lam. cv Tainon 65). It had a molecular weight of 250,000 and could be composed of five identical subunits. The isoelectric point of the
Versatile high-resolution oligosaccharide microarrays for plant glycobiology and cell wall research
Pedersen H L, et al.
The Journal of biological chemistry, M112-M112 (2012)
Maria Kylä-Puhju et al.
Meat science, 69(1), 143-149 (2005-01-01)
The activity of glycogen debranching enzyme (GDE) was studied in relation to pH value and temperature in porcine masseter and longissimus dorsi muscles. A glycogen limit dextrin was used as the substrate for GDE, and the enzyme was derived from
The phosphorylase b to a converting enzyme of rabbit skeletal muscle.
E G KREBS et al.
Biochimica et biophysica acta, 20(1), 150-157 (1956-04-01)
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