MBD0015
4′,6-diamidino-2-fenilindolo
For Nuclear counterstain in immunoflourecence microscopy, High Content Screening (HCS), Chromosome staining and flow cytometry (FACS)., 1 mg/mL
Sinonimo/i:
2-(4-amidinofenil)-6-indolocarbammidina, DAPI
Autenticatiper visualizzare i prezzi riservati alla tua organizzazione & contrattuali
About This Item
Formula empirica (notazione di Hill):
C16H15N5 · 2HCl
Numero CAS:
Peso molecolare:
350.25
Beilstein:
4894417
Numero MDL:
Codice UNSPSC:
41105500
NACRES:
NA.55
Prodotti consigliati
Livello qualitativo
Forma fisica
liquid
Condizioni di stoccaggio
protect from light
Concentrazione
1 mg/mL
tecniche
transfection: suitable
Solubilità
water: soluble
Fluorescenza
λex 340 nm; λem 488 nm (nur DAPI)
λex 364 nm; λem 454 nm (DAPI-DNA-Komplex)
Compatibilità
suitable for nucleic acid staining
Condizioni di spedizione
dry ice
Temperatura di conservazione
−20°C
Stringa SMILE
Cl.Cl.NC(=N)c1ccc(cc1)-c2cc3ccc(cc3[nH]2)C(N)=N
InChI
1S/C16H15N5.2ClH/c17-15(18)10-3-1-9(2-4-10)13-7-11-5-6-12(16(19)20)8-14(11)21-13;;/h1-8,21H,(H3,17,18)(H3,19,20);2*1H
FPNZBYLXNYPRLR-UHFFFAOYSA-N
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Descrizione generale
DAPI, 4′,6-diamidino-2-phenylindole, is a blue fluorescent nucleic acid stain that fluoresces brightly upon selectively binding to the minor groove of double stranded DNA. Its selectivity for DNA and high cell permeability allows efficient staining of nuclei with little background from the cytoplasm. The excitation and emission wavelengths of DAPI-DNA complex are 360 nm and 460 nm, respectively.
Applicazioni
DAPI è parecchie volte più sensibile del bromuro di etidio per colorare il DNA in gel di agarosio. Può essere impiegato per fotoimpronte del DNA, rivelare sonde ibridizzate in applicazioni blotting visualizzando specificamente il complesso a doppio filamento, studiare i cambiamenti nel DNA e analizzare con la citometria di flusso il contenuto di DNA durante l′apoptosi. La colorazione via DAPI si è dimostrata un metodo di rivelazione sensibile e specifico dei micoplasma.
DAPI Readymade Solution is provided at 1mg/mL concentration. It is recommended to dilute DAPI readymade solution 1:1000 with a desired buffer for a 1μg/mL working concentration (typical working concentration used in most applications is 0.1μg/mL-10μg/mL). Store DAPI solution (1mg/mL) -20°C protected from light. It is recommended to aliquot DAPI readymade solution and to avoid freeze thaw cycles.
DAPI is several times more sensitive than ethidium bromide for staining DNA in agarose gels. It may be used for photofootprinting of DNA, to detect annealed probes in blotting applications by specifically visualizing the double-stranded complex, and to study the changes in DNA and analyze DNA content during apoptosis using flow cytometry. DAPI staining has also been shown to be a sensitive and specific detection method for mycoplasma.
For Nuclear counterstain in immunoflourecence microscopy, High Content Screening (HCS), Chromosome staining and flow cytometry (FACS).
DAPI is several times more sensitive than ethidium bromide for staining DNA in agarose gels. It may be used for photofootprinting of DNA, to detect annealed probes in blotting applications by specifically visualizing the double-stranded complex, and to study the changes in DNA and analyze DNA content during apoptosis using flow cytometry. DAPI staining has also been shown to be a sensitive and specific detection method for mycoplasma.
For Nuclear counterstain in immunoflourecence microscopy, High Content Screening (HCS), Chromosome staining and flow cytometry (FACS).
Azioni biochim/fisiol
Sonda fluorescente per DNA permeabile alle cellule che si attacca al solco minore. Si attacca al solco minore del DNA a doppio filamento (preferenzialmente a DNA ricco di T) a formare un complesso stabile con una fluorescenza circa 20 volte maggiore del DAPI da solo.
Cell permeable fluorescent minor groove-binding probe for DNA. Binds to the minor groove of double-stranded DNA (preferentially to AT rich DNA), forming a stable complex which fluoresces approximately 20 times greater than DAPI alone.
Caratteristiche e vantaggi
- Ready-to-use liquid, cell permeable dye.
- Can be used with live cells and fixed cells.
- Effective for fixed-cell staining and quantitation of DNA content.
- Optimal Counterstain when using fluorescent antibodies during fluorescence microscopy or high-content screening (HCS) applications.
Stato fisico
DAPI readymade solution is provided at 1mg/mL concentration in water
Avvertenze
Warning
Indicazioni di pericolo
Consigli di prudenza
Classi di pericolo
Skin Sens. 1
Codice della classe di stoccaggio
12 - Non Combustible Liquids
Classe di pericolosità dell'acqua (WGK)
WGK 1
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
Certificati d'analisi (COA)
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