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Key Documents

MBD0013

Sigma-Aldrich

Microbial DNA standard from Escherichia coli

Suitable for PCR, sequencing and NGS, 10 ng/μL

Sinonimo/i:

E.Coli

Autenticatiper visualizzare i prezzi riservati alla tua organizzazione & contrattuali


About This Item

Codice UNSPSC:
41105500
NACRES:
NA.51

Livello qualitativo

Forma fisica

liquid

Concentrazione

10 ng/μL

tecniche

DNA extraction: suitable
DNA sequencing: suitable
PCR: suitable

Compatibilità

suitable for restriction endonuclease digests, PCR amplification, Southern blots, and sequencing reactions

Condizioni di spedizione

ambient

Temperatura di conservazione

−20°C

Descrizione generale

Standardization of sample analysis is currently needed in microbiome genomics research workflow. Lack of standardization can lead to biases and errors in common processes during sample preparation and analysis such as sample amplification, sequencing and bioinformatics analyses. Escherichia coli genomic DNA microbial standard can serve as standard for benchmarking the performance along the workflow of microbiomics or meta-genomics analyses and as a tool to increase reproducibility and allow comparison of results obtained by different labs. Escherichia coli is a gram negative, facultative aerobic, rod-shaped coliform bacterium. E. coli colonizes the infant gut within hours of birth and establishes itself as the most abundant facultative anaerobe of the human intestinal microflora for the remainder of life, equipped with the abilities to grow in the ever-changing environment in the gut and cope with the mammalian host interaction. Nevertheless, E. coli can survive in many different ecological habitats, including abiotic environments, and is considered a highly versatile species. Known habitats of E. coli include soil, water, sediment, and food. Some strains of E. coli have evolved and adapted to a pathogenic lifestyle and can cause different disease pathologies.


Read here how to use our standards to ensure data integrity for your microbiome research.

Applicazioni

The genomic DNA is provided at >=10 ng/μL concentration in TE buffer pH 8.0. It is recommended to avoid freeze thaw cycles of this product.
Suitable for Quantitative standard for PCR, Sequencing and NGS

Caratteristiche e vantaggi

  • Individual microbial standard for microbiomics and meta-genomics workflow
  • Suitable standard for PCR, sequencing and NGS
  • Improve Bioinformatics analyses
  • Increases reproducibility
  • Compare results lab to lab

Stato fisico

Liquid - The genomic DNA is provided at >=10 ng/μL concentration in TE buffer pH 8.0

Altre note

Strain: MG1655

Codice della classe di stoccaggio

12 - Non Combustible Liquids

Classe di pericolosità dell'acqua (WGK)

WGK 1

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable


Certificati d'analisi (COA)

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Order and disorder during Escherichia coli divergence.
Heather Hendrickson
PLoS genetics, 5(1), e1000335-e1000335 (2009-01-24)
Lisa C Crossman et al.
Journal of bacteriology, 192(21), 5822-5831 (2010-08-31)
In most cases, Escherichia coli exists as a harmless commensal organism, but it may on occasion cause intestinal and/or extraintestinal disease. Enterotoxigenic E. coli (ETEC) is the predominant cause of E. coli-mediated diarrhea in the developing world and is responsible
James B Kaper et al.
Nature reviews. Microbiology, 2(2), 123-140 (2004-03-26)
Few microorganisms are as versatile as Escherichia coli. An important member of the normal intestinal microflora of humans and other mammals, E. coli has also been widely exploited as a cloning host in recombinant DNA technology. But E. coli is
J Paul Brooks et al.
BMC microbiology, 15, 66-66 (2015-04-17)
Characterizing microbial communities via next-generation sequencing is subject to a number of pitfalls involving sample processing. The observed community composition can be a severe distortion of the quantities of bacteria actually present in the microbiome, hampering analysis and threatening the

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