M2414
Terreno essenziale minimo di Eagle
With Earle′s salts and reduced NaHCO₃. without ʟ-glutamine, liquid, sterile-filtered, suitable for cell culture
Sinonimo/i:
MEM
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About This Item
Codice UNSPSC:
12352207
NACRES:
NA.75
Prodotti consigliati
product name
Terreno essenziale minimo di Eagle, Modified, with Earle′s salts and reduced NaHCO3. without L-glutamine, liquid, sterile-filtered, suitable for cell culture
Livello qualitativo
Sterilità
sterile-filtered
Forma fisica
liquid
tecniche
cell culture | mammalian: suitable
Impurezze
endotoxin, tested
Componenti
phenol red: 0.011 g/L
NaHCO3: 0.85 g/L
glucose: 1.0 g/L
L-glutamine: no
Condizioni di spedizione
ambient
Temperatura di conservazione
2-8°C
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Descrizione generale
Minimum Essential Medium (MEM), developed by Harry Eagle, is one of the most widely used of all synthetic cell culture media. Early attempts to cultivate normal mammalian fibroblasts and certain subtypes of HeLa cells revealed they had specific nutritional requirements that could not be met by Eagle′s Basal Medium (BME). Subsequent studies using these and other cells in culture indicated additions to BME could be made to aid growth of a wider variety of fastidious cells.
MEM, which incorporates these modifications, includes higher concentrations of amino acids so the medium more closely approximates the protein composition of cultured mammalian cells. MEM has been used for cultivation of a wide variety of cells grown in monolayers. Optional supplementation of non-essential amino acids to the formulations that incorporate either Hanks′ or Earle′s salts has broadened the usefulness of this medium.
MEM, which incorporates these modifications, includes higher concentrations of amino acids so the medium more closely approximates the protein composition of cultured mammalian cells. MEM has been used for cultivation of a wide variety of cells grown in monolayers. Optional supplementation of non-essential amino acids to the formulations that incorporate either Hanks′ or Earle′s salts has broadened the usefulness of this medium.
Applicazioni
Recommended for use in a carbon dioxide-free culture system.
Altre note
This product lacks L-Ala; L-Asn; L-Glu; Gly; L-Pro; L-Ser and L-Gln.
May also be supplemented with HEPES to raise the buffering range.
Ricostituzione
Supplement with 0.292 g/L L-glutamine.
Comunemente ordinati con questo prodotto
N° Catalogo
Descrizione
Determinazione del prezzo
Supplemento
Codice della classe di stoccaggio
12 - Non Combustible Liquids
Classe di pericolosità dell'acqua (WGK)
WGK 1
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
Certificati d'analisi (COA)
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