L9641

Lipopolysaccharides (rough strains) from Escherichia coli EH100 (Ra mutant)

• Sinonimo/i: LPS
• Numero CE: 297-473-0
• Numero MDL: MFCD00164401
• Codice UNSPSC: 12352201
• NACRES: NA.25

Proprietà

• Origine biologica: Escherichia coli (EH100 Ra mutant)
• Livello qualitativo: 200
• Forma fisica: lyophilized powder
• Impurezze: <3% Protein (Lowry)
• Colore: white to yellow
• Solubilità: water: 4.90-5.10 mg/mL, cloudy to turbid, colorless to faintly yellow
• Condizioni di spedizione: ambient
• Temperatura di conservazione: 2-8°C

Descrizione

Descrizione generale

This product is phenol:chloroform:petroleum ether extracted from E. coli EH 100 (Ra mutant). The source strain is from a private collection.

Azioni biochim/fisiol

Lipopolysaccharides (LPS) are localized in the outer layer of the membrane and are, in noncapsulated strains, exposed on the cell surface. They contribute to the integrity of the outer membrane, and protect the cell against the action of bile salts and lipophilic antibiotics.

Nota sulla preparazione

Prepared by phenol-chloroform-petroleum ether extraction.

The product is soluble in water (5 mg/ml) or cell culture medium (1 mg/ml) yielding a hazy, faint yellow solution. A more concentrated, though still hazy, solution (20 mg/ml) has been achieved in aqueous saline after vortexing and warming to 70-80 ^oC. Lipopolysaccharides are molecules that form micelles in every solvent. Hazy solutions are observed in water and phosphate buffered saline. Organic solvents do not give clearer solutions. Methanol yields a turbid suspension with floaters, while water yields a homogeneously hazy solution.

Altre note

To gain a comprehensive understanding of our extensive range of Lipopolysaccharides for your research, we encourage you to visit our Carbohydrates Category page.

Informazioni sulla sicurezza

• Pittogrammi: GHS06
• Avvertenze: Danger
• Indicazioni di pericolo: H300
• Consigli di prudenza: P264 - P270 - P301 + P310 - P405 - P501
• Classi di pericolo: Acute Tox. 2 Oral
• Codice della classe di stoccaggio: 6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
• Classe di pericolosità dell'acqua (WGK): WGK 3
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable
• Dispositivi di protezione individuale: Eyeshields, Gloves, type N95 (US)