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Key Documents

L8795

Sigma-Aldrich

LB Agar Tetracycline-10, Plates

pre-poured agar plates with 10 μg/ml tetracycline

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About This Item

Codice UNSPSC:
12352200

Grado

for molecular biology

Livello qualitativo

100

Sterilità

sterile; autoclaved

Forma fisica

plates (10cm, 20-25ml each)

Disponibilità

available only in USA

Temperatura di conservazione

2-8°C

Compatibilità

selective for Escherichia coli
selective for coliforms

Descrizione generale

Miller′s LB is a highly-referenced microbial growth medium used for the cultivation of E. coli. This nutrient-rich microbial broth contains peptides, amino acids, water-soluble vitamins, and carbohydrates in a low-salt formulation. The addition of agar provides a solid medium for microbial growth.

Applicazioni

Suitable for selective cultivation of E. coli strains that contain plasmids conferring tetracycline resistance. The bacteria can be used for DNA plasmid production or production of recombinant proteins.

Caratteristiche e vantaggi

Miller′s LB Agar plates provide:
  • Convenient, ready-to-use 10cm format
  • Standard formulation plus antibiotic

Componenti

15g/L Agar
10g/L Tryptone
10g/L NaCl
5g/L Yeast Extract
10μg/ml tetracycline

Altre note

The plates must be used within two months of the manufacture date to ensure the antibiotics are fully active. The product may be shipped up until its expiration date but is generally shipped 2-4 weeks before expiration.

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LB Broth with agar (Miller), Highly-referenced nutrient-rich microbial growth powder medium with Agar, suitable for regular E.coli culture.

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Codice della classe di stoccaggio

10 - Combustible liquids

Classe di pericolosità dell'acqua (WGK)

WGK 1

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

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LB Broth with agar (Miller) Highly-referenced nutrient-rich microbial growth powder medium with Agar, suitable for regular E.coli culture.

Sigma-Aldrich

L3147

LB Broth with agar (Miller)

  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  4. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  5. Why do the LB-agar plates with antibiotics have such a short expiration date?

    The antibiotics in the LB-agar plates are not stable.  The antibiotic degrades over time, making it ineffective at selecting transformed bacteria grown on the plate.  Because of this, we recommend the plates are used as soon as possible once they are received.  For labs with low use, the plates may expire quickly, so they may benefit from pouring their own plates using the LB - Miller powder with agar formulation (L3147).

  6. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

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