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L5037

Sigma-Aldrich

Leptin from rat

≥97% (SDS-PAGE), recombinant, expressed in E. coli, lyophilized powder

Sinonimo/i:

OB

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About This Item

Numero MDL:
Codice UNSPSC:
51111800
NACRES:
NA.32
Origine biologica:
Escherichia coli
Stato:
lyophilized powder
Saggio:
≥97% (SDS-PAGE)

Origine biologica

Escherichia coli

Livello qualitativo

Ricombinante

expressed in E. coli

Sterilità

non-sterile

Saggio

≥97% (SDS-PAGE)

Stato

lyophilized powder

Potenza

≤0.8 ng/mL ED50

PM

16 kDa

Impurezze

≤1.0 EU/μg endotoxin

N° accesso UniProt

Condizioni di spedizione

ambient

Temperatura di conservazione

−20°C

Informazioni sul gene

rat ... Lep(25608)

Applicazioni

Leptin from rat has been used:
  • to check the effect of intranasal nerve growth factor (NGF) on NGF activity in the spinal cord of Sprague-Dawley rats
  • for validation of labelling for leptin-bearing cells of Sprague-Dawley rats
  • to study the effect of leptin on STAT3 (signal transducer and activator of transcription 3) phosphorylation in neural population of Sprague-Dawley rats
  • to study leptin response towards chemoreflex in nucleus of the solitary tract
  • in rats, to check the effect of association between leptin and neuronal nitric oxide pathway on penicillin-induced epileptiform activity

Azioni biochim/fisiol

Hormone produced primarily in adipocytes; primary site of action appears to be on neurons in the hypothalamus that are involved in regulating energy balance, appetite, and body weight.
In rodents with uncontrolled diabetes mellitus, leptin is crucial for normalizing hyperglycemia. In the digestive tract, it also participates in the absorption of dietary molecules via controlling transporters activity.
Leptin is a hormone produced primarily in adipocytes, although leptin mRNA has also been identified in placenta and fetal tissues, gastric tissue and liver. Its primary site of action appears to be on neurons in the hypothalamus that are involved in regulating energy balance, appetite, and body weight. Leptin increases the production of nitric oxide in endothelial cells and stimulates angiogenesis in vitro and in vivo.
Human and mouse leptin share ~84% sequence identity.

Stato fisico

Lyophilized from a 0.2 μm filtered solution with 4.5 mM NaHCO3.

Risultati analitici

The biological activity is measured by its ability to induce proliferation of leptin-dependent human OB-R transfected murine BAF3 cells.

Codice della classe di stoccaggio

11 - Combustible Solids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Dispositivi di protezione individuale

Eyeshields, Gloves, type N95 (US)


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John Ciriello et al.
American journal of physiology. Regulatory, integrative and comparative physiology, 303(7), R727-R736 (2012-08-24)
Circulating levels of leptin are elevated in individuals suffering from chronic intermittent hypoxia (CIH). Systemic and central administration of leptin elicits increases in sympathetic nervous activity (SNA), arterial pressure (AP), and heart rate (HR), and it attenuates the baroreceptor reflex
Iris A McDuffie et al.
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 52(2), 263-273 (2004-01-20)
Leptin, the ob protein, regulates food intake and satiety and can be found in the anterior pituitary. Leptin antigens and mRNA were studied in the anterior pituitary (AP) cells of male and female rats to learn more about its regulation.
Luigi Aloe et al.
Neural regeneration research, 9(10), 1025-1030 (2014-09-11)
The purpose of this work was to investigate whether, by intranasal administration, the nerve growth factor bypasses the blood-brain barrier and turns over the spinal cord neurons and if such therapeutic approach could be of value in the treatment of
Ali Aslan et al.
Brain research, 1321, 117-124 (2010-02-02)
The aim of the present study was to investigate the role of NO as a mediator of leptin action at the penicillin-induced epileptiform activity in rat. Thirty minutes after penicillin injection, leptin, at a dose of 1 microg, significantly increased
Carmen Fanjul et al.
Journal of physiology and biochemistry, 71(2), 311-317 (2015-05-04)
Leptin is secreted into the digestive tract and contributes to the absorption of dietary molecules by regulating transporters activity. Here, we studied the effect of luminal leptin on the intestinal transport of L-glutamate, an important component of human diet. We

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