PMSF-RO

Fenil metansolfonile fluoruro

Phenylmethylsulfonyl fluoride

• Sinonimo/i: αP-toluensolfonile fluoruro, Benzilsolfonile fluoruro, Fenil metilsolfonile fluoruro, PMSF
• Formula empirica (notazione di Hill): C₇H₇FO₂S
• Numero CAS: 329-98-6
• Peso molecolare: 174.19
• Beilstein: 2088311
• Numero MDL: MFCD00007424
• Codice UNSPSC: 12352202
• ID PubChem: 329823637

Proprietà

• Livello qualitativo: 100
• Saggio: 98% (TLC)
• Forma fisica: solid
• PM: 174.2
• Confezionamento: pkg of 10 g (10837091001); pkg of 25 g (11359061001)
• Produttore/marchio commerciale: Roche
• Concentrazione: 17-170 μg/mL
• tecniche: western blot: suitable
• pKa: 7.0
• Punto di fusione: 92-94 °C
• Solubilità: >10 mg/mL
• Condizioni di spedizione: ambient
• Temperatura di conservazione: 20-25°C
• Stringa SMILE: FS(=O)(=O)Cc1ccccc1
• InChI: 1S/C7H7FO2S/c8-11(9,10)6-7-4-2-1-3-5-7/h1-5H,6H2; YBYRMVIVWMBXKQ-UHFFFAOYSA-N

Descrizione

Descrizione generale

Soluble to >10 mg/ml in isopropanol, ethanol, methanol, or 1,2-propanediol. Unstable in aqueous solution. In 100% isopropanol, stable for at least 9 months at +25°C.

Specificità

Inhibits serine proteases such as chymotrypsin, trypsin, and thrombin, and the cysteine protease papain (reversible by DTT treatment). Does not inhibit metalloproteases, most cysteine proteases, or aspartic proteases.

Applicazioni

PMSF (C7H7O2SF) inhibits serine proteases such as chymotrypsin, trypsin, thrombin, and the cysteine protease papain (reversible by DTT treatment). It does not inhibit metalloproteases, most cysteine proteases, or aspartic proteases.
PMSF (phenylmethylsulfonyl fluoride) has been used for the preparation of protein extracts from tissues and cells prior to Western blotting.

Azioni biochim/fisiol

PMSF (phenylmethylsulfonyl fluoride) functions as a non-specific inhibitor of multiple proteases and other enzymes, such as palmityl coenzyme A deacylase, arylsulfatase A, acetylcholinesterase, trypsin and chymotrypsin. It is an irreversible inhibitor, and sulfonylates hydroxyl groups of the serine residues present in the active site of enzymes.

Qualità

Purity: Chromatographically homogeneous

Nota sulla preparazione

Activator: PK requires Mg²⁺ (or Mn²⁺, CO²⁺) and K⁺ (or NH⁴⁺, Rb⁺) for full activity.
Working concentration: 17 to 170 μg/ml (0.1 to 1 mM)
Working solution: Due to the short half-life of PMSF, fresh solution must be added at every purification step. Under certain conditions, such as alkaline pH values, PMSF may have a half-life of only 35 minutes. High-salt conditions (3 M), often used to precipitate proteins, may also reverse the inhibition of PMSF. Pefabloc SC is recommended as a non-toxic, water-soluble, and water-stable alternative.
Storage conditions (working solution): 15 to 25 °C
Stock solutions of PMSF in 100% isopropanol are stable for at least 9 months at 15 to 25 °C.

Ricostituzione

Soluble to > 10 mg/ml in isopropanol, ethanol, methanol, or 1,2-propanediol. Unstable in aqueous solution. In 100% isopropanol, stable for at least 9 months at 15 to 25 °C.

Altre note

Due to a short half-life, fresh solution must be added at every purification step. Under certain conditions, such as alkaline pH values, PMSF may only have a half-life of 35 min. High-salt conditions (3 M), often used to precipitate proteins, may also reverse the inhibition of PMSF. Pefabloc SC is recommended as a non-toxic, water-soluble, and water-stable alternative.

For life science research only. Not for use in diagnostic procedures.