PMSF-RO
Roche
Fenil metansolfonile fluoruro
Phenylmethylsulfonyl fluoride
Sinonimo/i:
αP-toluensolfonile fluoruro, Benzilsolfonile fluoruro, Fenil metilsolfonile fluoruro, PMSF
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About This Item
Formula empirica (notazione di Hill):
C7H7FO2S
Numero CAS:
Peso molecolare:
174.19
Beilstein:
2088311
Numero MDL:
Codice UNSPSC:
12352202
ID PubChem:
Prodotti consigliati
Livello qualitativo
Saggio
98% (TLC)
Forma fisica
solid
PM
174.2
Confezionamento
pkg of 10 g (10837091001)
pkg of 25 g (11359061001)
Produttore/marchio commerciale
Roche
Concentrazione
17-170 μg/mL
tecniche
western blot: suitable
pKa
7.0
Punto di fusione
92-94 °C
Solubilità
>10 mg/mL
Condizioni di spedizione
ambient
Temperatura di conservazione
20-25°C
Stringa SMILE
FS(=O)(=O)Cc1ccccc1
InChI
1S/C7H7FO2S/c8-11(9,10)6-7-4-2-1-3-5-7/h1-5H,6H2
YBYRMVIVWMBXKQ-UHFFFAOYSA-N
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Descrizione generale
Soluble to >10 mg/ml in isopropanol, ethanol, methanol, or 1,2-propanediol. Unstable in aqueous solution. In 100% isopropanol, stable for at least 9 months at +25°C.
Specificità
Inhibits serine proteases such as chymotrypsin, trypsin, and thrombin, and the cysteine protease papain (reversible by DTT treatment). Does not inhibit metalloproteases, most cysteine proteases, or aspartic proteases.
Applicazioni
PMSF (C7H7O2SF) inhibits serine proteases such as chymotrypsin, trypsin, thrombin, and the cysteine protease papain (reversible by DTT treatment). It does not inhibit metalloproteases, most cysteine proteases, or aspartic proteases.
PMSF (phenylmethylsulfonyl fluoride) has been used for the preparation of protein extracts from tissues and cells prior to Western blotting.
PMSF (phenylmethylsulfonyl fluoride) has been used for the preparation of protein extracts from tissues and cells prior to Western blotting.
Azioni biochim/fisiol
PMSF (phenylmethylsulfonyl fluoride) functions as a non-specific inhibitor of multiple proteases and other enzymes, such as palmityl coenzyme A deacylase, arylsulfatase A, acetylcholinesterase, trypsin and chymotrypsin. It is an irreversible inhibitor, and sulfonylates hydroxyl groups of the serine residues present in the active site of enzymes.
Qualità
Purity: Chromatographically homogeneous
Nota sulla preparazione
Activator: PK requires Mg2+ (or Mn2+, CO2+) and K+ (or NH4+, Rb+) for full activity.
Working concentration: 17 to 170 μg/ml (0.1 to 1 mM)
Working solution: Due to the short half-life of PMSF, fresh solution must be added at every purification step. Under certain conditions, such as alkaline pH values, PMSF may have a half-life of only 35 minutes. High-salt conditions (3 M), often used to precipitate proteins, may also reverse the inhibition of PMSF. Pefabloc SC is recommended as a non-toxic, water-soluble, and water-stable alternative.
Storage conditions (working solution): 15 to 25 °C
Stock solutions of PMSF in 100% isopropanol are stable for at least 9 months at 15 to 25 °C.
Working concentration: 17 to 170 μg/ml (0.1 to 1 mM)
Working solution: Due to the short half-life of PMSF, fresh solution must be added at every purification step. Under certain conditions, such as alkaline pH values, PMSF may have a half-life of only 35 minutes. High-salt conditions (3 M), often used to precipitate proteins, may also reverse the inhibition of PMSF. Pefabloc SC is recommended as a non-toxic, water-soluble, and water-stable alternative.
Storage conditions (working solution): 15 to 25 °C
Stock solutions of PMSF in 100% isopropanol are stable for at least 9 months at 15 to 25 °C.
Ricostituzione
Soluble to > 10 mg/ml in isopropanol, ethanol, methanol, or 1,2-propanediol. Unstable in aqueous solution. In 100% isopropanol, stable for at least 9 months at 15 to 25 °C.
Altre note
Due to a short half-life, fresh solution must be added at every purification step. Under certain conditions, such as alkaline pH values, PMSF may only have a half-life of 35 min. High-salt conditions (3 M), often used to precipitate proteins, may also reverse the inhibition of PMSF. Pefabloc SC is recommended as a non-toxic, water-soluble, and water-stable alternative.
For life science research only. Not for use in diagnostic procedures.
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