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K4002

Sigma-Aldrich

Krebs-Ringer Bicarbonate Buffer

With 1800 mg/L glucose, without calcium chloride and sodium bicarbonate, powder, suitable for cell culture

Sinonimo/i:

Bicarbonate Buffer Solution, Krebs Buffer, Krebs-Ringer Solution

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About This Item

Codice UNSPSC:
12161702
NACRES:
NA.75

Livello qualitativo

Stato

powder

tecniche

cell culture | mammalian: suitable

pH

6.1-6.7

Solubilità

water: 9.5 g/L, clear

Temperatura di conservazione

2-8°C

Descrizione generale

Krebs-Ringer Bicarbonate Buffer acts as a salt solution and plays an essential role in tissue culture. A salt solution provides the cells with water and inorganic ions. It also helps in maintaining the osmotic balance and ideal pH in the medium. This bicarbonate buffered isotonic saline contains magnesium, potassium, sodium, and phosphate ions.

Applicazioni

Krebs-Ringer Bicarbonate Buffer has been used:

  • as a wash buffer for the preparation of mice primary adipocytes and lipolysis assay
  • in the pre-perifusion for islet viability and functionality studies
  • in pituitary bioassay

Quantità

Formulated to contain 9.5 grams of powder per liter of medium.

Ricostituzione

Supplement with 1.26 g/L sodium bicarbonate.

Codice della classe di stoccaggio

11 - Combustible Solids

Classe di pericolosità dell'acqua (WGK)

WGK 1

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable


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Protocolli

Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.

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