K1757

Keratinocyte Growth Factor human

≥95% (SDS-PAGE and HPLC), recombinant, expressed in E. coli, powder, suitable for cell culture

• Sinonimo/i: hFGF-7, hKGF, FGF-7, KGF
• Numero CAS: 148348-15-6
• Numero MDL: MFCD00212245
• Codice UNSPSC: 12352202
• NACRES: NA.77

Proprietà

• product name: Keratinocyte Growth Factor human, KGF, recombinant, expressed in E. coli, powder, suitable for cell culture
• Origine biologica: human
• Livello qualitativo: 200
• Ricombinante: expressed in E. coli
• Saggio: ≥95% (SDS-PAGE and HPLC)
• Forma fisica: powder
• Potenza: 0.1-15 ng/mL ED₅₀/EC₅₀
• Qualità: endotoxin tested
• PM: 18.9 kDa
• Confezionamento: pkg of 10 and 50 μg
• tecniche: cell culture | mammalian: suitable
• Impurezze: 1 EU/μg
• N° accesso UniProt: P21781
• Temperatura di conservazione: −20°C
• Informazioni sul gene: human ... FGF7(2252)

Descrizione

Descrizione generale

Keratinocyte Growth Factor(KGF) is a 18.9 kDa growth factor, spanning 163 amino acids, belongs to the fibroblast growth factor family. It includes a secretory signal sequence and shares 39% identity with FGF-Basic. It is expressed in a number of stromal fibroblast cell lines, mesenchymal cells but absent from normal glial cells and epithelial cell lines.

Applicazioni

Keratinocyte Growth Factor human has been used:

• as a component of epithelial growth medium
• as a positive control for antigen to determine the activity of the antibody
• to analyze the influence of KGF associated with thermo-sterilized carboxymethyl chitosan (NOCCts) in the reduction of pericardial adhesions

Azioni biochim/fisiol

Keratinocyte Growth Factor(KGF) stimulates proliferation and differentiation of keratinocytes and other cells in the epithelium. It induces chemotaxis of microvascular endothelial cells and neovascularization of rat cornea. It stimulates the hyaluronic acid synthesis in proliferating and migrating basal cells. KGF contributes to the survival of cells under stress conditions. KGF is highly expressed in injured and inflamed epithelial cells, thereby, promotes wound healing.

Stato fisico

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline containing 0.5 mg bovine serum albumin.

Risultati analitici

Determined by a cell proliferation assay using 4MBr-5 cells.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 11 - Combustible Solids
• Classe di pericolosità dell'acqua (WGK): WGK 3
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable
• Dispositivi di protezione individuale: dust mask type N95 (US), Eyeshields, Gloves