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K0253

Sigma-Aldrich

Keratin from human epidermis

aqueous solution

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About This Item

Numero CAS:
Numero MDL:
Codice UNSPSC:
12352202
NACRES:
NA.61

Origine biologica

human epidermis

Livello qualitativo

Saggio

≥90% (SDS-PAGE)

Stato

urea solution

tecniche

ELISA: suitable

N° accesso UniProt

Temperatura di conservazione

2-8°C

Informazioni sul gene

human ... KRT1(3848)

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Descrizione generale

Keratins are characteristic intermediate filament proteins of the epithelia. In human, keratin is classified into type I and type II. About 54 genes encodes for the keratin protein. The keratin 1 (KRT1) gene is mapped to human chromosome 12q13.13. It is the primary component of hair, skin and nails.

Applicazioni

Keratin from human epidermis has been used:
  • as an analyte and a cross-reactant in the antibody-immobilized chip for testing selectivity coefficients
  • as a standard in extracellular matrix (ECM) protein in in vitro protein degradation assays.
  • asa a standard in enzyme linked immunosorbent assay (ELISA)

Azioni biochim/fisiol

Keratins are important for the mechanical stability and integrity of epithelial cells and tissues. Some keratins also have regulatory functions and are involved in intracellular signaling pathways, e.g. protection from stress, wound healing, and apoptosis. This diverse group of cytoskeletal scaffolding proteins form intermediate filament networks which provide structural support to keratinocytes that maintain the integrity of the skin. Expression of keratin genes is usually regulated by differentiation of the epidermal cells within the stratifying squamous epithelium.

Stato fisico

Solution in 8 M urea, 50 mM Tris, 0.1 M β-mercaptoethanol and 0.1% sodium azide, pH 8.4

Pittogrammi

Exclamation mark

Avvertenze

Warning

Indicazioni di pericolo

Classi di pericolo

Skin Sens. 1

Codice della classe di stoccaggio

10 - Combustible liquids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable


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Cornelia Kröger et al.
The Journal of cell biology, 201(5), 681-692 (2013-05-22)
Maintenance of epithelial cell adhesion is crucial for epidermal morphogenesis and homeostasis and relies predominantly on the interaction of keratins with desmosomes. Although the importance of desmosomes to epidermal coherence and keratin organization is well established, the significance of keratins
Keratin-based Biomaterials and Bioproducts, 5-5 (2017)
[Sinonasal neoplasms].
Zhan-bo Wang et al.
Zhonghua bing li xue za zhi = Chinese journal of pathology, 42(1), 50-51 (2013-04-25)
Comparison of biomarkers in exhaled breath condensate and bronchoalveolar lavage
Jackson AS, et al.
American Journal of Respiratory and Critical Care Medicine, 175(3), 222-227 (2007)
Christian Mozet et al.
Anticancer research, 33(6), 2415-2424 (2013-06-12)
The hedgehog signaling pathway (HH) is involved in tumorigenesis in a variety of human malignancies. In head and neck squamous cell carcinomas (HNSCC), Hh overexpression was associated with poor prognosis. Therefore, we analyzed the effect of Hh signaling blockade with

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