Passa al contenuto
Merck
Tutte le immagini(1)

Documenti

CC076

Sigma-Aldrich

Collagene di topo, tipo IV:

Autenticatiper visualizzare i prezzi riservati alla tua organizzazione & contrattuali
Tutte le immagini(1)

About This Item

Codice UNSPSC:
12352202
eCl@ss:
32160405
NACRES:
NA.75

Origine biologica

human

Livello qualitativo

100

Saggio

95% (Human collagen type IV, SDS-PAGE)

Forma fisica

liquid

PM

300 kDa

Produttore/marchio commerciale

Chemicon®

Concentrazione

1 mg/mL

tecniche

cell culture | mammalian: suitable

Impurezze

<5% Human collagen types I-III, V, VI, and non-collagen proteins

input

sample type epithelial cells
sample type hematopoietic stem cell(s)
sample type pancreatic stem cell(s)
sample type neural stem cell(s)
sample type mesenchymal stem cell(s)
sample type induced pluripotent stem cell(s)
sample type: human embryonic stem cell(s)

Solubilità

water: soluble at 20 °C

N° accesso NCBI

NM_001845.4

N° accesso UniProt

P02462

Specificità del legame

Peptide Source: Laminin

Peptide Source: Nidogen

Condizioni di spedizione

dry ice

Temperatura di conservazione

−20°C

Informazioni sul gene

human ... COL4A1(1282)

Categorie correlate

Descrizione generale

Collagen type IV is the major structural element of basal membranes. Human collagen type IV is a native triple helix. Collagen was extracted from washed dissected tissue into dilute acetic acid after mild pepsin treatment. The Collagen type IV was purified by using differential salt precipitation. Purity and retention of native helical structure was controlled by SDS-PAGE and by reaction with anti-collagen type specific monoclonal antibodies. Peso molecolare: 300kDa; Solubility: 10mg/mL
Origine del prodotto: Human placenta, negative for HBsAg and HIV antibodies

Applicazioni





Stato fisico

Liquid purified protein. μ Salt-free, no preservative.

Stoccaggio e stabilità

Conservare a -20ºC per un massimo di 12 mesi. Evitare ripetuti cicli di congelamento e scongelamento.

Risultati analitici

Retention of native structure was confirmed by ability to form microfibrils.

Note legali

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Esclusione di responsabilità

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

Codice della classe di stoccaggio

12 - Non Combustible Liquids

Classe di pericolosità dell'acqua (WGK)

WGK 1

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

Possiedi già questo prodotto?

I documenti relativi ai prodotti acquistati recentemente sono disponibili nell’Archivio dei documenti.

Visita l’Archivio dei documenti

Isolation and partial characterization of a new human collagen with an extended triple-helical structural domain.
Bentz, H, et al.
Proceedings of the National Academy of Sciences of the USA, 80, 3168-3172 (1983)
Characterization of a novel collagen chain in human placenta and its relation to AB collagen.
Sage, H and Bornstein, P
Biochemistry, 18, 3815-3822 (1979)
R W Glanville et al.
European journal of biochemistry, 95(2), 383-389 (1979-04-02)
Native type IV collagen was isolated from human placenta using pepsin solubilisation followed by fractional salt precipitation and chromatogarphic purification. The native preparation was characterised using amino acid analyses, disc gel electrophoresis, segment-long-spacing crystallites and immunological methods. Two component alpha

Articoli

Extracellular Matrix Proteins and Tools for Cell Culture Optimization

Extracellular matrix proteins such as laminin, collagen, and fibronectin can be used as cell attachment substrates in cell culture.

Contenuto correlato

ECM Coating Protocols for Millicell®

This page covers the ECM coating protocols developed for four types of ECMs on Millicell®-CM inserts, Collagen Type 1, Fibronectin, Laminin, and Matrigel.

Il team dei nostri ricercatori vanta grande esperienza in tutte le aree della ricerca quali Life Science, scienza dei materiali, sintesi chimica, cromatografia, discipline analitiche, ecc..

Contatta l'Assistenza Tecnica.