H5041
Cystatin C human
recombinant, expressed in HEK 293 cells, HumanKine®, suitable for cell culture
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About This Item
Prodotti consigliati
Origine biologica
human
Livello qualitativo
Ricombinante
expressed in HEK 293 cells
Saggio
≥95% (SDS-PAGE)
Forma fisica
lyophilized powder
Potenza
≤5 μM IC50
Qualità
endotoxin tested
PM
dimer 12-13 kDa (non-glycosylated)
Confezionamento
pkg of 10 μg
Condizioni di stoccaggio
avoid repeated freeze/thaw cycles
tecniche
cell culture | mammalian: suitable
Impurezze
≤1 EU/mg
N° accesso UniProt
Temperatura di conservazione
−20°C
Informazioni sul gene
human ... CYTC(1471)
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Descrizione generale
HumanKine human Cystatin C is expressed as a non-glycosylated monomer in human HEK 293 cells. Cystatin C belongs to the cystatin superfamily. Two isoforms (pI = 7.8, 9.2) of native Cystatin C are found in human urine differentiated by the elimination of small basic peptides or amino acids from the N-terminal end of the protein. This protein is coded by a housekeeping gene, CST3 located on human chromosome 20p11.21 and is generated by nucleated cells.
Applicazioni
Cystatin C human has been used as a standard in western blot assay. It has also been used to study its effect on the endopeptidase activity of activated Trichobilharzia regenti isoform of cathepsin B1 peptidase (TrCB1).
Azioni biochim/fisiol
Cystatin C is an inhibitor of cysteine proteases including cathepsin B which has been identified as the most important β-amyloid-degrading enzyme. Measurement of cystatin C in serum is replacing creatinine as an indicator of kidney function (glomerular filtration rate, GFR). Studies show its role in predicting new-onset or deteriorating cardiovascular disease. It also seems to play a role in brain disorders involving amyloid, such as Alzheimer′s disease. Cystatin C inhibits transforming growth factor β signaling in normal and cancer cells.
Stato fisico
Lyophilized from a 0.2 μm filtered solution of 1×PBS
Risultati analitici
The inhibitory function of cystatin c on papain′s protease activity was measured by a colorimetric assay using L-BAPA as substrate. IC50 value was measured at 5 to 20 μg/mL (0.3 to 1.5 μM) with a range of 1.56 μg/mL to 50 μg/mL cystatin C in presence of 0.55 μM papain and 0.44 μM L-BAPA.
Note legali
HumanKine is a registered trademark of Proteintech Group, Inc. and Humanzyme, Inc
Codice della classe di stoccaggio
11 - Combustible Solids
Classe di pericolosità dell'acqua (WGK)
WGK 3
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
Certificati d'analisi (COA)
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