GERPN6302
Iodoacetamide
Cytiva RPN6302, pack of 25 g
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About This Item
Prodotti consigliati
Stato
solid
Caratteristiche
wetted part: no
Confezionamento
pack of 25 g
Produttore/marchio commerciale
Cytiva RPN6302
Colore
Yellowish
Solubilità
cold water: soluble (Easily soluble in cold and hot water)
Temperatura di conservazione
2-8°C
Categorie correlate
Descrizione generale
Used for the efficient alkylation of thiols while minimizing oxidation of competing thiol pairs in protein samples.
Applicazioni
Iodoacetamide has been used:
- as a component in urea-sodium dodecyl sulfate (SDS) buffer for yeast cell lysis in immunoprecipitation assay
- in equilibration of pH gradient strips during the second dimension run in isoelectric focusing
- as a protein alkylation agent
Azioni biochim/fisiol
Iodoacetamide (IAA) modifies thiol groups in proteins by S-carboxyamidomethylation. Iodoacetamide is an alkylating agent. IAA is commonly used to analyze the key role of cysteine residues in the catalytic reactions of enzymes. It is also used in the derivatization of cysteine residues in proteins for proteomic analysis. IAA irreversibly inhibits glyceraldehyde-3-phosphate dehydrogenase (GAPDH).
Caratteristiche e vantaggi
- Ultrapure, proteomic-grade iodoacetamide is used for efficient alkylation of thiols while minimizing oxidation of the competing thiol pairs in protein samples.
Stoccaggio e stabilità
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Risultati analitici
To view the Certificate of Analysis for this product, please visit www.cytiva.com.
Avvertenze
Danger
Indicazioni di pericolo
Consigli di prudenza
Codice della classe di stoccaggio
6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects
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Protocolli
Rehydration Solutions
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