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GE28-4082-53

HiTrap® MabSelect™

Cytiva 28-4082-53, pack of 5 × 1 mL

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About This Item

Codice UNSPSC:
23151816
NACRES:
NA.56

ligand

recombinant protein A (E. coli)

Confezionamento

pack of 5 × 1 mL

Produttore/marchio commerciale

Cytiva 28-4082-53

Condizioni di stoccaggio

(20% Ehtanol)
 (20% Ethanol)

Parametri

42 psi

Dimensioni del letto

7 mm × 25 mm

Volume del letto

1 mL

Diam. int. colonna

7 mm

Matrice

highly cross-linked agarose

Dimens. media delle particelle

85 μm (d₅₀v is the median particle size of the cumulative volume distribution)

cleaning

2-12

working range

3-10

Capacità

≥30 mg binding capacity (humanlgG/ml at 2.4 min residence time)

Compatibilità

suitable for bioprocess medium

Temperatura di conservazione

2-8°C

Descrizione generale

Determined at 10% breakthrough by frontal analysis at a mobile phase velocity of 500 cm/h in a column with a bed height of 20 cm.
HiTrap® MabSelect™ columns are prepacked with MabSelect, a Bioprocess medium for capuring of Mabs from large sample volumes.
pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.

Applicazioni

Lab Purification using the MabSelect™ Protein A tag for capture of antibodies.

Caratteristiche e vantaggi

  • HiTrap® columns prepacked with MabSelect, a Bioprocess medium for capuring of Mabs from large sample volumes. • The specificity of binding to the Fc region of IgG is similar to that of native protein A, which allows one-step purification.
  • Oriented coupling of recombinant Protein A to the matrix via an engineered C-terminal cysteine enhances IgG binding capacity.
  • Fewer regulatory concerns due to the total absence of mammalian culture in the ligand production and purification.

Stoccaggio e stabilità

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Risultati analitici

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Altre note

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

Note legali

HiTrap is a registered trademark of Cytiva
MabSelect is a trademark of Cytiva

Prodotti correlati

N° Catalogo
Descrizione
Determinazione del prezzo

Pittogrammi

Flame
GHS02

Avvertenze

Warning

Indicazioni di pericolo

H226

Codice della classe di stoccaggio

3 - Flammable liquids

Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

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Articoli

Column Packing and Preparation for Affinity Chromatography of Antibodies

This page describes efficient column packing and preparation for affinity chromatography of antibodies.

Desalting and Buffer Exchange for Affinity Chromatography of Antibodies

This page shows how to perform sample desalting and buffer exchange for affinity chromatography of antibodies.

Affinity Chromatography Troubleshooting

This page shows how to solve practical problems that may occur when running an affinity chromatography column.

Protocolli

Performing a Separation with GE Healthcare Products Based on Protein A

Protein A is derived from a strain of Staphylococcus aureus and contains five regions that bind to the Fc region of IgG. As an affinity ligand, protein A is coupled to Sepharose so that these regions are free to bind IgG.

Converting From Linear Fow (cm/hour) to Volumetric Flow Rates (mL/min) and Vice Versa

This page shows how to convert between linear flow and volumetric flow rates in affinity chromatography.

Buffer Exchange and Desalting for Affinity Chromatography

How to perform buffer exchange and desalting with Sephadex G-25, HiTrap Desalting columns, or ÄKTAprime plus.

Column Packing and Preparation Protocol for Affinity Chromatography

This page shows how to perform column packing and preparation for affinity chromatography when using Tricorn™ or XK columns available from GE Healthcare.

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