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Documenti fondamentali

GE17-0963-03

Protein A Sepharose™ Cl-4B

Cytiva 17-0963-03, pack of 25 mL

Sinonimo/i:

Protein A Column, Sepharose 4B

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About This Item

Codice UNSPSC:
41106500
NACRES:
NA.56

ligand

native protein A

Confezionamento

pack of 25 mL

Produttore/marchio commerciale

Cytiva 17-0963-03

Condizioni di stoccaggio

(20% Ehtanol)

Matrice

4% cross-linked agarose

Dimensione particelle

45-165 μm

Diametro medio

90 μm

cleaning

2-10

Intervallo di esercizio

3-9

Capacità

~40 mg binding capacity (histidine-tagged protein/ml medium when changed with Ni2+)(Protein- and metal-ion dependent.)

Temperatura di conservazione

2-8°C

Descrizione generale

Protein A Sepharose™ CL-4B is a classic, well documented medium for immunoglobin purification and fractionation, and is suitable for immunoprecipitation procedures.

Protein A binds to the Fc region of immunoglobulins through interactions with the heavy chain. The binding of protein A has been well documented for IgG from a variety of mammalian species and for some IgM and IgA as well. Protein A Sepharose™ CL-4B has been used as a powerful tool to isolate and purify classes, subclasses and fragments of immunoglobulins from biological fluids and from cell culture media. Since only the Fc region is involved in binding, the Fab region is available for binding antigen.
pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.

Caratteristiche e vantaggi

  • Well documented for immunoglobulin purification and fractionation
  • Strong binding to Fc region of IgG
  • High capacity - 20 mg human IgG/mL.
  • Suited for immunoprecipitation procedures

Stoccaggio e stabilità

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Risultati analitici

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Note legali

Sepharose is a trademark of Cytiva

Pittogrammi

Flame
GHS02

Avvertenze

Warning

Indicazioni di pericolo

H226

Codice della classe di stoccaggio

3 - Flammable liquids

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