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Key Documents

GE17-0090-02

Sephadex® LH-20

Cytiva 17-0090-02, pack of 500 g

Sinonimo/i:

Sephadex Gel

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About This Item

Codice UNSPSC:
23151817
NACRES:
NA.56

Caratteristiche

autoclavable 20 min at 121°C

Confezionamento

pack of 500 g

Produttore/marchio commerciale

Cytiva 17-0090-02

Matrice

hydroxypropylated, cross-linked dextran

Dimensione particelle

163 μm (in methanol max.)
18-111 μm (dry)
27 μm (In Methanol Min.)

cleaning in place

2-13

working range

2-13

Descrizione generale

Sephadex® LH20 is a liquid chromatography media for molecular sizing of natural product such as steroid, terpenoid, lipid, low molecular weight peptide. Useful to analytical and industrial scale for preparation of closely related molecular species.

Sephadex® LH-20 is prepared by hydroxypropylation of Sephadex® G-25, a bead-formed dextran medium, and has been specifically developed for gel filtration of natural products, such as steroids, terpenoids, lipids and low molecular weight peptides, in organic solvents.

Sephadex® LH-20 is useful at both analytical and industrial scale for the preparation of closely related molecular species. Due to the physico-chemical properties of this media, it can be used either during initial purification prior to polishing by high performance ion exchange or reversed phase chromatography or as the final polishing step e.g. during the preparation of diastereomers. Sephadex® LH-20 is characterized by (1) Chromatographic selectivity due to dual hydrophilic and lipophilic nature of the matrix (2) Easily predicted elution behaviour based on the chemical structure 1–3, 16 of the sample (3) Chemical and physical robustness (4) Excellent batch to batch reproducibility.

Caratteristiche e vantaggi

  • Designed for use in polar organic solvents and aqueous solvent mixtures
  • Excellent for fractionation of lipids, hormones, vitamins, and other small biomolecules.
  • Useful for both analytical and industrial scales for the preparation of closely related molecular species
  • Sephadex® LH-20 is made from hydroxypropylated dextran beads that have been crosslinked to yield a polysaccharide network.
  • Powerful chromatographic selectivity due to dual hydrophilic and lipophilic nature of the matrix
  • Excellent batch-to-batch reproducibility
  • Sephadex® LH-20 is made from hydroxypropylated dextran beads that have been crosslinked to yield a polysaccharide network.

Stoccaggio e stabilità

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 25 °C ()

Risultati analitici

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Note legali

Sephadex is a registered trademark of Cytiva

Codice della classe di stoccaggio

13 - Non Combustible Solids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable


Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

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Protocolli

This page shows how to perform a separation with a Sephadex LH-20 column from GE Healthcare which are Size Exclusion Chromatography media of polysaccharide network made from cross-linked hydroxypropylated dextran beads.

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