G9269

Anti-Glial Fibrillary Acidic Protein Antibody

rabbit polyclonal

• Sinonimo/i: GFAP Antibody Sigma - Anti-Glial Fibrillary Acidic Protein antibody produced in rabbit, Gfap Antibody Sigma, Anti-GFAP
• Numero MDL: MFCD00145904
• Codice UNSPSC: 12352203
• NACRES: NA.41

Proprietà

• Nome del prodotto: Anti-Glial Fibrillary Acidic Protein antibody produced in rabbit, IgG fraction of antiserum, buffered aqueous solution
• Origine biologica: rabbit
• Livello qualitativo: 200
• Coniugato: unconjugated
• Forma dell’anticorpo: IgG fraction of antiserum
• Tipo di anticorpo: primary antibodies
• Clone: polyclonal
• Stato: buffered aqueous solution
• Reattività contro le specie: rat, human
• Confezionamento: antibody small pack of 25 μL
• tecniche: immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:80 using brain sections; microarray: suitable; western blot: 1:500 using brain extract
• N° accesso UniProt: P14136
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: −20°C
• modifica post-traduzionali bersaglio: unmodified
• Informazioni sul gene: human ... GFAP(2670) ; rat ... Gfap(24387)

Descrizione

Descrizione generale

The GFAP (glial fibrillary acidic protein) gene encodes an intermediate protein filament-III (50kDa). It is localized particularly to the astrocytes and non-myelinating Schwann cells of the central and peripheral nervous system, respectively.

Specificità

The product reacts specifically with GFAP and labels astrocytes, glial cells and glial cell derived tumors in immunohistochemical staining. The antibody reacts with glial specific antigen in alcohol or formalin-fixed, paraffin-embedded human or animal tissue sections.

Immunogeno

GFAP from human brain

Applicazioni

Anti-Glial Fibrillary Acidic Protein antibody produced in rabbit is suitable for:

• immunohistochemical staining at a working dilution of 1:400 using rat brain sections
• immunohistochemical staining of human brain tumor tissue specimens to identify the cells expressing AQP4 (Aquaporin-4)
• immunofluorescence using brain section from mice

The antibody is suitable for immunohistochemistry at a working dilution of 1:80 using alcohol- and formalin-fixed, paraffin-embedded sections of human or animal brain tissue. It is also suitable for indirect immunoblotting at a working dilution of 1:500 using human brain extract blot and microarray.

Azioni biochim/fisiol

Glial fibrillary acidic protein may be used as a marker for distinguishing astrocytes from other glial cells during development of the central nervous system. GFAP is responsible for maintaining the structure of glial cells. It also offers mechanical strength and support to the surrounding neurons and blood brain barrier. Defects in this gene causes Alexander disease, indicated by Rosenthal fibers deposition. It is a rare disorder of astrocytes in the central nervous system. Increased GFAP expression indicates astrogliosis, a sign of neurodegeneration and neuroinflammation.

Stato fisico

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide

Stoccaggio e stabilità

Store at 2-8 °C up to one month. For extended storage, solution may be frozen in working aliquots. Repeated freezing and thawing, or storage in "frost-free" freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify by centrifugation before use.

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 12 - Non Combustible Liquids
• Classe di pericolosità dell'acqua (WGK): WGK 2
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable