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G8255

Sigma-Aldrich

Glutamic-Pyruvic Transaminase from porcine heart

lyophilized powder, ≥75 units/mg protein

Sinonimo/i:

L-Alanine: 2-Oxoglutarate Aminotransferase, Alanine Aminotransferase (catalytic concentration), GPT

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About This Item

Numero CAS:
9000-86-6
Classificazione EC (Enzyme Commission):
2.6.1.2 (BRENDA, IUBMB)
Beilstein:
3562140
Numero CE:
232-561-4
Numero MDL:
MFCD00131192
Codice UNSPSC:
12352204
NACRES:
NA.54

Origine biologica

Porcine heart

Livello qualitativo

200

Forma fisica

lyophilized powder

Attività specifica

≥75 units/mg protein

Composizione

Protein, 75-95%

tecniche

cell culture | mammalian: suitable

Colore

light yellow to yellow

Attività estranea

L-glutamic dehydrogenase, lactic dehydrogenase and malic dehydrogenase ≤0.01%
glutamic-oxalacetic transaminase ≤0.1%

Temperatura di conservazione

−20°C

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Descrizione generale

Glutamic-pyruvic transaminase (GPT) is localized majorly in the liver.

Research area: Cell Signaling

Applicazioni

Glutamic-Pyruvic Transaminase from porcine heart has been used:
  • in the transamination assay with a racemic mixture of synthetic 3-methyl-2-oxoglutarate
  • to remove glutamate from the cell culture medium used for culturing human embryonic kidney (HEK293) cells
  • in the lactate-dehydrogenase (LD)-coupled alanine transaminase (ALT) reaction kinetic studies

Azioni biochim/fisiol

Glutamic-pyruvic transaminase (GPT) mediates the transfer of the amino group to α-ketoglutarate from l-alanine which results in pyruvate and glutamate.Thereby GPT plays a key role in gluconeogenesis and amino acid metabolism. The role of GPT in various kinds of tumors has been reported.
Glutamic-pyruvic transaminase (GPT) is located in most cells in the body, but is found in highest concentrations in the liver. It is used as a marker of liver disease due to the release of GPT into the blood following liver cell necrosis.

Definizione di unità

One unit will convert 1.0 μmole of α-ketoglutarate to L-glutamate per min at pH 7.6 at 37 °C in the presence of L-alanine.

Stato fisico

Lyophilized powder containing citrate and acetate

Stoccaggio e stabilità

Tightly closed. Dry. Keep locked up or in an area accessible only to qualified or authorized
persons

Risultati analitici

Protein determined by biuret

Pittogrammi

Health hazard
GHS08

Avvertenze

Danger

Indicazioni di pericolo

H334

Consigli di prudenza

P261 - P284 - P501

Classi di pericolo

Resp. Sens. 1

Codice della classe di stoccaggio

11 - Combustible Solids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Dispositivi di protezione individuale

Eyeshields, Gloves, type N95 (US)

Certificati d'analisi (COA)

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Giordano Pula et al.
Journal of biochemical and biophysical methods, 64(3), 167-181 (2005-08-23)
The surface expression of G protein-coupled receptors is regulated by internalization. For many receptors, a constitutive level of internalization in the absence of agonist has been reported. The constitutive internalization of metabotropic glutamate receptor 1a (mGluR1a) has been described, but
A simple and sensitive detection of glutamic-pyruvic transaminase activity based on fluorescence quenching of bovine serum albumin
Chen M, et al.
Royal Society of Chemistry Advances, 5, 103557-103562 (2015)
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